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Blood, 15 November 2005, Vol. 106, No. 10, pp. 3650-3657. Prepublished online as a Blood First Edition Paper on August 4, 2005; DOI 10.1182/blood-2005-02-0582. This Article Full Text (PDF) All Versions of this Article: 2005-02-0582v1 106/10/3650    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wang, L. Articles by Rowe, D. W Search for Related Content PubMed PubMed Citation Articles by Wang, L. Articles by Rowe, D. W Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted February 10, 2005 Accepted July 15, 2005 Heterogeneity of engrafted bone lining cells after systemic and local transplantation Liping Wang, Yaling Liu, Zana Kalajzic, Xi Jiang, and David W Rowe* Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, CT, USA * Corresponding author; email: rowe{at}neuron.uchc.edu. The outcome of various osteoprogenitor cell transplantation protocols was assessed using Col1a1-GFP reporter transgenic mice. The model requires the recipient mice undergo lethal total body irradiation (TBI) followed by rescue with whole bone marrow. When the mice are rescued with total bone marrow from a Col1a1-GFP transgenic mouse, GFP positive donor cells can be observed on most endosteal and trabecular bone surfaces. Although the cells express an osteoblast-restricted GFP, they fail to progress to osteocytes, do not form a mineralized matrix and do not generate bone nodules in vitro. However when calvarial progenitor cells derived from the same transgenic mice are injected into the bone marrow space, osteogenesis by the donor cells is observed. Using different GFP colors that distinguish the donor and recipient osteoblasts, commingling of the two cells types is observed along the mineralizing osteoblast surface as well as within the osteocyte population of the endosteal bone. Despite the ability of the injected progenitor cells to produce bone within the injected bone, they lack the ability to form mineralized bone nodules when explanted to primary osteoblast culture. These reagents and imaging protocols will be useful in evaluating other cells having a better progenitor potential than calvarial derived stromal cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. Morikawa, Y. Mabuchi, Y. Kubota, Y. Nagai, K. Niibe, E. Hiratsu, S. Suzuki, C. Miyauchi-Hara, N. Nagoshi, T. Sunabori, et al. Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow J. Exp. Med., October 26, 2009; 206(11): 2483 - 2496. [Abstract] [Full Text] [PDF]

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