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Blood, 15 October 2005, Vol. 106, No. 8, pp. 2781-2789. Prepublished online as a Blood First Edition Paper on July 5, 2005; DOI 10.1182/blood-2005-02-0597. This Article Full Text (PDF) All Versions of this Article: 2005-02-0597v1 106/8/2781    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Renaudineau, Y. Articles by Youinou, P. Search for Related Content PubMed PubMed Citation Articles by Renaudineau, Y. Articles by Youinou, P. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted February 11, 2005 Accepted April 27, 2005 An alternative exon 1 of the cd5 gene regulates CD5 expression in human B lymphocytes Yves Renaudineau, Sophie Hillion, Alain Saraux, Rizgar A Mageed, and Pierre Youinou* Laboratory of Immunology, Brest University Medical School, Brest, France William Harvey Research Institute, Queen Mary School of Medicine, London, United Kingdom * Corresponding author; email: youinou{at}univ-brest.fr. T lymphocytes and a subpopulation of B lymphocytes express the CD5 co-receptor. Its functional importance is evident from the multiple levels and developmental stages of the regulation of its expression. We report the discovery of a novel regulatory exon upstream of the non-coding region of the cd5 gene in humans. This alternate exon 1 is designated E1B (with the conventional exon 1 renamed E1A), and shown to regulate the expression of CD5. E1B-containing transcripts exist exclusively in B lymphocytes, encode a protein which is truncated, and retained intracellularly. As a consequence, the amount of E1A-containing transcripts is down-regulated, and the membrane CD5 expression diminished in the presence of E1B-containing transcripts. High levels of E1A-transcripts were found in chronic lymphocytic leukemia, and there were no E1A-transcripts in 697 pre-B cells which have no membrane CD5. Introduc-tion of E1B into Jurkat cells reduces their membrane expression of CD5, and sequence analysis reveals that the E1B motif is a defective human endogenous retrovirus. A balance between the 2 alternative exons 1 might be central to the regulation of membrane CD5 in human B cells, and, through CD5-associated SH2-containing phosphatase 1, to the modulation of B cell antigen receptor-transduced signals. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. Garaud, C. Le Dantec, S. Jousse-Joulin, C. Hanrotel-Saliou, A. Saraux, R. A. Mageed, P. Youinou, and Y. Renaudineau IL-6 Modulates CD5 Expression in B Cells from Patients with Lupus by Regulating DNA Methylation J. Immunol., May 1, 2009; 182(9): 5623 - 5632. [Abstract] [Full Text] [PDF] N. Shani, H. Rubin-Lifshitz, Y. Peretz-Cohen, K. Shkolnik, V. Shinder, M. Cohen-Sfady, Y. Shav-Tal, M. Barda-Saad, and D. Zipori Incomplete T-cell receptor-{beta} peptides target the mitochondrion and induce apoptosis Blood, April 9, 2009; 113(15): 3530 - 3541. [Abstract] [Full Text] [PDF] S. Garaud, C. Le Dantec, C. Berthou, P. M. Lydyard, P. Youinou, and Y. Renaudineau Selection of the Alternative Exon 1 from the cd5 Gene Down-Regulates Membrane Level of the Protein in B Lymphocytes J. Immunol., August 1, 2008; 181(3): 2010 - 2018. [Abstract] [Full Text] [PDF] R. Williams Stay Away From Self: 5th International Congress on Autoimmunity, Sorrento, Italy, November 29-December 3, 2006 J. Exp. Med., March 19, 2007; 204(3): 456 - 457. [Full Text] [PDF]

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