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Blood, 15 July 2005, Vol. 106, No. 2, pp. 514-520.
Prepublished online as a Blood First Edition Paper on April 5, 2005; DOI 10.1182/blood-2005-02-0598.
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Submitted February 11, 2005
Accepted March 22, 2005
Zygotic nucleosome assembly protein like 1 has a specific, non-cell autonomous role in hematopoiesis
Anita Abu-Daya, Wendy M Steer, Alexandra F Trollope, Christine E Friedeberg, Roger K Patient, Alan W Thorne, and Matthew J Guille*
School of Biological Sciences, Institute of Biomedical and Biomolecular Sciences, University of Portsmouth, Portsmouth, United Kingdom
Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, United Kingdom
* Corresponding author; email: matthew.guille{at}port.ac.uk.
Nucleosome assembly proteins (NAPs) bind core histones, facilitate chromatin remodeling and can act as transcriptional co-activators. We previously described the isolation of a Xenopus NAP1L cDNA, which encodes a member of this protein family. Its zygotic expression is restricted to neural cells, the outer cells of the ventral blood islands (VBI) and the ectoderm overlying the blood precursors. Here we report that depletion of zygotic xNAP1L in embryos produces no obvious morphological phenotype, but ablates  -globin mRNA expression in the VBI. Transcript levels of the haematopoietic precursor genes SCL and Xaml (Runx-1) are also reduced in the VBI. SCL expression can be rescued by injection of xNAP1L mRNA into the ectoderm, showing that the effect of xNAP1L can be non-cell autonomous. Fli1 and Hex, genes expressed in haemangioblasts but subsequently endothelial markers, were unaffected suggesting that xNAP1L is required for the haematopoietic lineage specifically. Our data are consistent with a requirement for xNAP1L upstream of SCL and injection of SCL mRNA into xNAP1L-depleted embryos rescues -globin expression. Thus xNAP1L, which belongs to a family of proteins previously believed to have general roles, has a specific function in haematopoiesis.
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