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Blood, 1 January 2006, Vol. 107, No. 1, pp. 184-186.
Prepublished online as a Blood First Edition Paper on September 15, 2005; DOI 10.1182/blood-2005-02-0647.
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Submitted February 15, 2005
Accepted September 2, 2005
Use of interleukin 7 receptor-alpha knockout donor cells demonstrates the lymphoid independence of dendritic cells
Satoshi Takeuchi and Stephen I Katz*
Dermatology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
* Corresponding author; email: katzs{at}od.niams.nih.gov.
The precise lineage of dendritic cells (DC), including skin Langerhans cells (LC), is unclear. Interleukin 7 (IL-7) and its receptor (IL-7R ) are known to mediate lymphopoiesis, and IL-7 is also known to be essential for the generation of DC from lymphoid committed precursors in vitro. Thus, to determine the developmental lymphoid (or IL-7R) dependency of various DCs and to examine the importance of IL-7/IL-7R for DC development in vivo, we utilized IL-7R knockout (KO) donor cells to reconstitute DCs/LC in sublethally irradiated recipients, and compared the results to those obtained using wild type (WT) donor cells. We found that lymphoid lineage cells (except NK cells), including thymocytes, were less efficiently reconstituted by IL-7R KO donor cells, while myeloid lineage cells and DCs/LC were equally well reconstituted by both the IL-7R KO and WT donor cells. Overall, we conclude that IL-7R is not required for the development of DCs/LC in vivo.
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