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Blood, 1 October 2005, Vol. 106, No. 7, pp. 2566-2571. Prepublished online as a Blood First Edition Paper on June 9, 2005; DOI 10.1182/blood-2005-02-0674. This Article Full Text (PDF) All Versions of this Article: 2005-02-0674v1 106/7/2566    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Basu, P. Articles by Lloyd, J. A Search for Related Content PubMed PubMed Citation Articles by Basu, P. Articles by Lloyd, J. A Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted February 17, 2005 Accepted May 26, 2005 KLF2 is essential for primitive erythropoiesis and regulates the human and murine embryonic -like globin genes in vivo Priyadarshi Basu, Pamela E Morris, Jack L Haar, Maqsood A Wani, Jerry B Lingrel, Karin M Gaensler, and Joyce A Lloyd* Department of Human Genetics, Virginia Commonwealth University, Richmond, Virginia, USA Department of Anatomy and Neurobiology, Virginia Commonwealth University, Richmond, Virginia, USA Department of Molecular Genetics, Biochemistry, and Microbiology, College of Medicine, University of Cincinnati, Cincinnati, Ohio, USA Department of Medicine, University of California, San Francisco, California, USA * Corresponding author; email: jlloyd{at}hsc.vcu.edu. The Kruppel-like factors (KLFs) are a family of C2/H2 zinc finger DNA binding proteins that are important in controlling developmental programs. Erythroid Kruppel-like Factor (EKLF or KLF1) positively regulates the -globin gene in definitive erythroid cells. KLF2 (LKLF) is closely related to EKLF and is expressed in erythroid cells. KLF2-/- mice die between E12.5 and E14.5, due to severe intra-embryonic hemorrhaging. They also display growth retardation and anemia. We investigated the expression of the -like globin genes in KLF2 knockout mice. Our results show that KLF2-/- mice have a significant reduction of murine embryonic Ey- and h1-globin but not -globin gene expression in the E10.5 yolk sac, compared to wild-type mice. The expression of the adult maj- and min-globin genes is unaffected in the fetal livers of E12.5 embryos. In mice carrying the entire human globin locus, KLF2 also regulates the expression of the human embryonic -globin gene but not the adult -globin gene, suggesting that this developmental stage-specific role is evolutionarily conserved. KLF2 also plays a role in the maturation and/or stability of erythroid cells in the yolk sac. KLF2-/- embryos have a significantly increased number of primitive erythroid cells undergoing apoptotic cell death. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: An unexpected entry into the globin real estate market James J. Bieker Blood 2005 106: 2230-2231. [Full Text] [PDF] This article has been cited by other articles: S. Y. Liang, B. Moghimi, V. J. Crusselle-Davis, I-J. Lin, M. H. Rosenberg, X. Li, J. Strouboulis, S. Huang, and J. Bungert Defective Erythropoiesis in Transgenic Mice Expressing Dominant-Negative Upstream Stimulatory Factor Mol. Cell. 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[Abstract] [Full Text] [PDF] S. A. Eaton, A. P. W. Funnell, N. Sue, H. Nicholas, R. C. M. Pearson, and M. Crossley A Network of Kruppel-like Factors (Klfs): Klf8 IS REPRESSED BY Klf3 AND ACTIVATED BY Klf1 IN VIVO J. Biol. Chem., October 3, 2008; 283(40): 26937 - 26947. [Abstract] [Full Text] [PDF] J. K. Alder, R. W. Georgantas III, R. L. Hildreth, I. M. Kaplan, S. Morisot, X. Yu, M. McDevitt, and C. I. Civin Kruppel-Like Factor 4 Is Essential for Inflammatory Monocyte Differentiation In Vivo J. Immunol., April 15, 2008; 180(8): 5645 - 5652. [Abstract] [Full Text] [PDF] C. T. Griffin, J. Brennan, and T. Magnuson The chromatin-remodeling enzyme BRG1 plays an essential role in primitive erythropoiesis and vascular development Development, February 1, 2008; 135(3): 493 - 500. [Abstract] [Full Text] [PDF] P. Basu, T. K. Lung, W. Lemsaddek, T. G. Sargent, D. C. Williams Jr, M. Basu, L. C. Redmond, J. B Lingrel, J. L. Haar, and J. A. Lloyd EKLF and KLF2 have compensatory roles in embryonic {beta}-globin gene expression and primitive erythropoiesis Blood, November 1, 2007; 110(9): 3417 - 3425. [Abstract] [Full Text] [PDF] M. Kobayashi and M. Yamamoto Regulation of GATA1 Gene Expression J. Biochem., July 1, 2007; 142(1): 1 - 10. [Abstract] [Full Text] [PDF] A. P. W. Funnell, C. A. Maloney, L. J. Thompson, J. Keys, M. Tallack, A. C. Perkins, and M. Crossley Erythroid Kruppel-Like Factor Directly Activates the Basic Kruppel-Like Factor Gene in Erythroid Cells Mol. Cell. Biol., April 1, 2007; 27(7): 2777 - 2790. [Abstract] [Full Text] [PDF] D. Zhou, K. M. Pawlik, J. Ren, C.-W. Sun, and T. M. Townes Differential Binding of Erythroid Krupple-like Factor to Embryonic/Fetal Globin Gene Promoters during Development J. Biol. Chem., June 9, 2006; 281(23): 16052 - 16057. [Abstract] [Full Text] [PDF] J. P. Huddleson, N. Ahmad, and J. B. Lingrel Up-regulation of the KLF2 Transcription Factor by Fluid Shear Stress Requires Nucleolin J. Biol. Chem., June 2, 2006; 281(22): 15121 - 15128. [Abstract] [Full Text] [PDF] A. M. Pilon, D. G. Nilson, D. Zhou, J. Sangerman, T. M. Townes, D. M. Bodine, and P. G. Gallagher Alterations in expression and chromatin configuration of the alpha hemoglobin-stabilizing protein gene in erythroid kruppel-like factor-deficient mice. Mol. Cell. Biol., June 1, 2006; 26(11): 4368 - 4377. [Abstract] [Full Text] [PDF] H. Das, A. Kumar, Z. Lin, W. D. Patino, P. M. Hwang, M. W. Feinberg, P. K. Majumder, and M. K. Jain Kruppel-like factor 2 (KLF2) regulates proinflammatory activation of monocytes PNAS, April 25, 2006; 103(17): 6653 - 6658. [Abstract] [Full Text] [PDF] D. Hodge, E. Coghill, J. Keys, T. Maguire, B. Hartmann, A. McDowall, M. Weiss, S. Grimmond, and A. Perkins A global role for EKLF in definitive and primitive erythropoiesis Blood, April 15, 2006; 107(8): 3359 - 3370. [Abstract] [Full Text] [PDF] N. Matsumoto, A. Kubo, H. Liu, K. Akita, F. Laub, F. Ramirez, G. Keller, and S. L. Friedman Developmental regulation of yolk sac hematopoiesis by Kruppel-like factor 6 Blood, February 15, 2006; 107(4): 1357 - 1365. [Abstract] [Full Text] [PDF] P. D. Kingsley, J. Malik, R. L. Emerson, T. P. Bushnell, K. E. McGrath, L. A. Bloedorn, M. Bulger, and J. Palis "Maturational" globin switching in primary primitive erythroid cells Blood, February 15, 2006; 107(4): 1665 - 1672. [Abstract] [Full Text] [PDF]

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