SEARCH:   Advanced

Blood, 15 November 2005, Vol. 106, No. 10, pp. 3440-3448. Prepublished online as a Blood First Edition Paper on August 9, 2005; DOI 10.1182/blood-2005-03-0857. This Article Full Text (PDF) Supplemental Figures All Versions of this Article: 2005-03-0857v1 106/10/3440    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sundrud, M. S Articles by Unutmaz, D. Search for Related Content PubMed PubMed Citation Articles by Sundrud, M. S Articles by Unutmaz, D. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted March 1, 2005 Accepted June 30, 2005 The transcription factor GATA-1 potently represses the expression of the HIV-1 co-receptor CCR5 in human T cells and dendritic cells Mark S Sundrud, Scott E VanCompernolle, Karla A Eger, Tullia C Bruno, Arun Subramaniam, Srinivas Mummidi, Sunil K Ahuja, and Derya Unutmaz* Department of Microbiology and Immunology, Vanderbilt University Medical School, Nashville, TN, USA Department of Internal Medicine, Sanofi Aventis Pharmaceuticals, Bridgewater, NJ, USA Veterans Administration Center for AIDS and HIV infection, South Texas Veterans Health Care System, and Department of Medicine, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA * Corresponding author; email: derya.unutmaz{at}vanderbilt.edu. CCR5 is the major HIV-1 co-receptor and its expression levels are a critical determinant of HIV-1 infection. However, the molecular mechanisms of CCR5 regulation in primary targets of HIV-1 remain unknown. Despite binding to conserved DNA-elements, we show that the transcription factors GATA-1 and GATA-3 differentially suppress the expression of CCR5 in stem cell-derived dendritic cells and primary human T cell subsets. In addition, GATA-1 expression was also more potent than GATA-3 in suppressing Th1 associated genes, IFN and CXCR3. GATA-1, but not GATA-3, potently suppressed CCR5 transcription, thereby rendering human T cells resistant to CCR5-tropic HIV-1 infection. However, GATA-1 also substituted for GATA-3 in its canonical role of programming Th2 gene expression. These findings provide insight into GATA-3-mediated gene regulation during T cell differentiation. Importantly, decoding the mechanisms of GATA-1-mediated repression of CCR5 may offer an opportunity to develop novel approaches to inhibit CCR5 expression in T cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: L. Gutierrez, T. Nikolic, T. B. van Dijk, H. Hammad, N. Vos, M. Willart, F. Grosveld, S. Philipsen, and B. N. Lambrecht Gata1 regulates dendritic-cell development and survival Blood, September 15, 2007; 110(6): 1933 - 1941. [Abstract] [Full Text] [PDF]

	Copyright © 2005 by American Society of Hematology         Online ISSN: 1528-0020