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Blood, 15 December 2005, Vol. 106, No. 13, pp. 4225-4233. Prepublished online as a Blood First Edition Paper on August 23, 2005; DOI 10.1182/blood-2005-03-0975. This Article Full Text (PDF) Supplemental Figures All Versions of this Article: 2005-03-0975v1 106/13/4225    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Haase, C. Articles by von Herrath, M. G Search for Related Content PubMed PubMed Citation Articles by Haase, C. Articles by von Herrath, M. G Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted March 10, 2005 Accepted August 11, 2005 Immunomodulatory dendritic cells require autologous serum to circumvent non-specific immunosuppressive activity in vivo Claus Haase, Mette Ejrnaes, Amy E Juedes, Tom Wolfe, Helle Markholst*, and Matthias G von Herrath Hagedorn Research Institute, Gentofte, Denmark; ImmunoPharmacology, Novo Nordisk A/S, Malov, Denmark La Jolla Institute for Allergy and Immunology, San Diego, CA, USA * Corresponding author; email: hmar{at}hagedorn.dk. In immunotherapy, dendritic cells (DCs) can be used as powerful antigen-presenting cells to enhance or suppress antigen-specific immunity upon in vivo transfer in mice or humans. However, to generate sufficient numbers of DCs most protocols include an ex vivo culture step, wherein the cells are exposed to heterologous serum and/or antigenic stimuli. In mouse models of virus infection and virus-induced autoimmunity, we tested how heterologous serum affects the immunomodulatory capacity of immature DCs generated in the presence of IL-10 by comparing FBS- or normal mouse serum (NMS)-supplemented DC-cultures. We show that FBS-exposed DCs induce a systemic immune deviation characterized by reduction of virus-specific T cells, delayed viral clearance and enhanced systemic produc-tion of IL-4, IL-5 and IL-10 to FBS-derived antigens, including BSA. By contrast, DCs generated in NMS-supplemented cultures modulated immunity and autoimmunity in an antigen-specific fashion. These cells did not induce systemic IL-4, IL-5 or IL-10 production and inhibited generation of virus-specific T-cells or autoimmunity only if pulsed with a viral antigen. These data underscore the importance of using autologous serum-derived immature DCs in preclinical animal studies to accurately assess their immunomodulatory potential in future human therapeutic settings, where application of FBS is not feasible. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: L. A. Novotny, S. Partida-Sanchez, R. S. Munson Jr., and L. O. Bakaletz Differential Uptake and Processing of a Haemophilus influenzae P5-Derived Immunogen by Chinchilla Dendritic Cells Infect. Immun., March 1, 2008; 76(3): 967 - 977. [Abstract] [Full Text] [PDF]

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