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Blood, 15 August 2005, Vol. 106, No. 4, pp. 1296-1304. Prepublished online as a Blood First Edition Paper on April 26, 2005; DOI 10.1182/blood-2005-03-0998. This Article Full Text (PDF) All Versions of this Article: 2005-03-0998v1 106/4/1296    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Hager-Theodorides, A. L Articles by Crompton, T. Search for Related Content PubMed PubMed Citation Articles by Hager-Theodorides, A. L Articles by Crompton, T. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted March 11, 2005 Accepted April 11, 2005 The transcription factor Gli3 regulates differentiation of fetal CD4-CD8-Double Negative thymocytes Ariadne L Hager-Theodorides, Johannes T Dessens, Susan V Outram, and Tessa Crompton* Division of Cell and Molecular Biology, Faculty of Life Sciences, Imperial College London, London, United Kingdom * Corresponding author; email: t.crompton{at}ic.ac.uk. Glioblastoma (Gli) 3 is a transcription factor involved in patterning and oncogenesis. Here we demonstrate a role for Gli3 in thymocyte development. Gli3 is differentially expressed in fetal CD4-CD8- double negative (DN) thymocytes, and is most highly expressed at the CD44+CD25-DN (DN1) and CD44-CD25- (DN4) stages of development, but was not detected in adult thymocytes. Analysis of null mutants showed that Gli3 is involved at the transitions from DN1 to CD44+CD25+DN (DN2) cell and from DN to CD4+CD8+ double positive (DP) cell. Gli3 is required for differentiation from DN to DP thymocyte, after pre-T Cell Receptor (TCR) signaling but is not necessary for pre-TCR induced proliferation or survival. The effect of Gli3 was dose-dependent, suggesting its direct involvement in the transcriptional regulation of genes controlling T cell differentiation during fetal development. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. L. Hager-Theodorides, A. L. Furmanski, S. E. Ross, S. V. Outram, N. J. Rowbotham, and T. Crompton The Gli3 Transcription Factor Expressed in the Thymus Stroma Controls Thymocyte Negative Selection Via Hedgehog-Dependent and -Independent Mechanisms J. Immunol., September 1, 2009; 183(5): 3023 - 3032. [Abstract] [Full Text] [PDF] N. J. Rowbotham, A. L. Hager-Theodorides, A. L. Furmanski, S. E. Ross, S. V. Outram, J. T. Dessens, and T. Crompton Sonic hedgehog negatively regulates pre-TCR-induced differentiation by a Gli2-dependent mechanism Blood, May 21, 2009; 113(21): 5144 - 5156. [Abstract] [Full Text] [PDF] S. V. Outram, A. L. Hager-Theodorides, D. K. Shah, N. J. Rowbotham, E. Drakopoulou, S. E. Ross, B. Lanske, J. T. Dessens, and T. Crompton Indian hedgehog (Ihh) both promotes and restricts thymocyte differentiation Blood, March 5, 2009; 113(10): 2217 - 2228. [Abstract] [Full Text] [PDF] A. Varas, C. Hernandez-Lopez, J. Valencia, S. Mattavelli, V. G. Martinez, L. Hidalgo, C. Gutierrez-Frias, A. G. Zapata, R. Sacedon, and A. Vicente Survival and function of human thymic dendritic cells are dependent on autocrine Hedgehog signaling J. Leukoc. Biol., June 1, 2008; 83(6): 1476 - 1483. [Abstract] [Full Text] [PDF] N. J. Rowbotham, A. L. Hager-Theodorides, M. Cebecauer, D. K. Shah, E. Drakopoulou, J. Dyson, S. V. Outram, and T. Crompton Activation of the Hedgehog signaling pathway in T-lineage cells inhibits TCR repertoire selection in the thymus and peripheral T-cell activation Blood, May 1, 2007; 109(9): 3757 - 3766. [Abstract] [Full Text] [PDF]

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