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Blood, 15 August 2005, Vol. 106, No. 4, pp. 1259-1261.
Prepublished online as a Blood First Edition Paper on May 5, 2005; DOI 10.1182/blood-2005-03-1081.
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Submitted March 17, 2005
Accepted April 19, 2005
Short-term repopulating stem cells are efficiently detected following intra-femoral transplantation into NOD/SCID recipients depleted of CD122+ cells
Joby L McKenzie, Olga I Gan, Monica Doedens, and John E Dick*
Department of Molecular and Medical Genetics, University of Toronto, Toronto, ON, Canada; Division of Cell and Molecular Biology, University Health Network, Toronto, ON, Canada
* Corresponding author; email: jdick{at}uhnres.utoronto.ca.
The NOD/SCID xenotransplant model has emerged as a widely employed assay for human hematopoietic stem cells, however, barriers still exist that limit engraftment. We previously identified a short-term SCID repopulating cell (SRC) following direct intrafemoral (IF) injection into NOD/SCID mice, whereas others characterized similar SRC using NOD/SCID mice depleted of NK cell activity. To determine the model that most efficiently detects short-term SRC, we compared human engraftment in six different xenotransplant models; NOD/SCID- 2 microglobulin-null mice, anti-CD122 (IL-2R) treated or unmanipulated NOD/SCID mice, each transplanted by intravenous or IF injection. Human cell engraftment was highest in IF injected anti-CD122 treated NOD/SCID mice compared to all other groups at 2 and 6 weeks post-transplant. These modifications to the SRC assay provide improved detection of human stem cells and demonstrate that CD122+ cells provide barriers to stem cell engraftment; a finding with potential clinical relevance.
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