SEARCH:   Advanced

Blood, 1 March 2006, Vol. 107, No. 5, pp. 1837-1846. Prepublished online as a Blood First Edition Paper on November 8, 2005; DOI 10.1182/blood-2005-03-1180. This Article Full Text (PDF) All Versions of this Article: 2005-03-1180v1 107/5/1837    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Karpatkin, S. Articles by Gupta, R. Search for Related Content PubMed PubMed Citation Articles by Karpatkin, S. Articles by Gupta, R. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted March 25, 2005 Accepted August 12, 2005 Hematopoiesis and stem cell renewal in long-term bone marrow cultures containing catalase Simon Karpatkin*, Ross Basch, and Rashmi Gupta NYU Cancer Institute, New York University School of Medicine, New York, NY, USA Department of Medicine, New York University School of Medicine, New York, NY, USA; NYU Cancer Institute, New York University School of Medicine, New York, NY, USA Department of Pathology, New York University School of Medicine, New York, NY, USA; Department of Medicine, New York University School of Medicine, New York, NY, USA * Corresponding author; email: simon.karpatkin{at}med.nyu.edu. Culturing mouse bone marrow in the presence of catalase dramatically alters hematopoiesis. Granulocyte output is initially increased 4-5 fold. This increase is transient and granulocyte production declines as immature (Sca-1+/LIN-) cells accumulate. One-third of these immature cells have a phenotype (Sca-1+/c-Kit +) characteristic of hematopoietic stem cells. At 2-3 weeks there are >200 fold more Sca-1+/c-Kit+/LIN- cells in treated cultures than in controls. This population contains functional stem cells with both short-term and long-term bone marrow repopulating activity. In addition to myeloid progenitors, this Sca-1+/LIN- population contain a large number of cells that express CD31, CD34, and have an active Tie-2 promoter, indicating that they are in the endothelial lineage. After 3-4 weeks hematopoiesis in treated cultures wanes but if catalase is removed, hematopoiesis resumes. After 7-10 days the cultures are indistinguishable from untreated controls. Thus, protected from H2O2, hematopoietic progenitors multiply and become quiescent. This sequence resembles in vivo development in normal marrow. These results make it clear that peroxide-sensitive regulatory mechanisms play an important role in controlling hematopoiesis ex vivo and presumably in vivo as well. They also indicate that manipulation of the peroxide levels can be used to enhance the growth of hematopoietic stem cells in culture. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

	Copyright © 2005 by American Society of Hematology         Online ISSN: 1528-0020