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Blood, 1 September 2005, Vol. 106, No. 5, pp. 1843-1850. Prepublished online as a Blood First Edition Paper on May 10, 2005; DOI 10.1182/blood-2005-03-1281. This Article Full Text (PDF) Supplemental Figures All Versions of this Article: 2005-03-1281v1 106/5/1843    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Abadie, V. Articles by Winter, N. Search for Related Content PubMed PubMed Citation Articles by Abadie, V. Articles by Winter, N. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted March 30, 2005 Accepted April 28, 2005 Neutrophils rapidly migrate via lymphatics after Mycobacterium bovis BCG intradermal vaccination and shuttle live bacilli to the draining lymph nodes Valerie Abadie, Edgar Badell, Patrice Douillard, Danielle Ensergueix, Pieter J Leenen, Myriam Tanguy, Laurence Fiette, Sem Saeland, Brigitte Gicquel, and Nathalie Winter* Mycobacterial Genetics Unit, Institut Pasteur, Paris, France Laboratory for Immunological Research, Schering Plough, Dardilly, France Department of Immunology, Erasmus Medical Center, Rotterdam, The Netherlands Laboratory of Histotechnology and Pathology, Institut Pasteur, Paris, France * Corresponding author; email: nwinter{at}pasteur.fr. The early innate response after Mycobacterium bovis BCG vaccination is poorly characterized, but probably decisive for subsequent protective immunity against tuberculosis. Therefore, we vaccinated mice with fluorescent BCG strains in the ear dorsum, as a surrogate of intradermal vaccination in humans. During the first three days, we tracked BCG host cells migrating out of the dermis to the auricular draining lymph nodes (ADLN). Resident skin dendritic cells (DCs) or macrophages did not play a predominant role in early BCG capture and transport to ADLN. The main BCG host cells rapidly recruited both in the dermis and ADLN were neutrophils. Fluorescent green or red BCG strains injected into non-overlapping sites were essentially sheltered by distinct neutrophils in the ADLN capsule indicating that neutrophils had captured bacilli in peripheral tissue and transported them to the lymphoid organ. Strikingly, we observed BCG-infected neutrophils in the lumen of lymphatic vessels by confocal microscopy on ear dermis. Fluorescent-labeled neutrophils injected into the ears, accumulated exclusively into the ipsilateral ADLN capsule after BCG vaccination. Thus, we provide in vivo evidence that neutrophils, like DCs or inflammatory monocytes, migrate via afferent lymphatics to lymphoid tissue and can shuttle live microorganisms. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. Tanne, B. Ma, F. Boudou, L. Tailleux, H. Botella, E. Badell, F. Levillain, M. E. Taylor, K. Drickamer, J. Nigou, et al. A murine DC-SIGN homologue contributes to early host defense against Mycobacterium tuberculosis J. Exp. Med., September 28, 2009; 206(10): 2205 - 2220. [Abstract] [Full Text] [PDF] A. G. Rothfuchs, J. G. Egen, C. G. Feng, L. R. V. Antonelli, A. Bafica, N. Winter, R. M. Locksley, and A. Sher In Situ IL-12/23p40 Production during Mycobacterial Infection Is Sustained by CD11bhigh Dendritic Cells Localized in Tissue Sites Distinct from Those Harboring Bacilli J. Immunol., June 1, 2009; 182(11): 6915 - 6925. [Abstract] [Full Text] [PDF] R. Evans, I. Patzak, L. 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