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Blood, 1 December 2005, Vol. 106, No. 12, pp. 3718-3724. Prepublished online as a Blood First Edition Paper on August 4, 2005; DOI 10.1182/blood-2005-04-1366. This Article Full Text (PDF) All Versions of this Article: 2005-04-1366v1 106/12/3718    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Gasser, O. Articles by Hess, C. Search for Related Content PubMed PubMed Citation Articles by Gasser, O. Articles by Hess, C. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted April 4, 2005 Accepted July 26, 2005 Human CD8+ T cells store CXCR1 in a distinct intracellular compartment and up-regulate it rapidly to the cell-surface upon activation Olivier Gasser, Anna Missiou, Ceylan Eken, and Christoph Hess* Department of Research, Immunobiology laboratory, University Hospital Basel, Basel, Switzerland * Corresponding author; email: chess{at}uhbs.ch. Activation and subsequent differentiation of naive CD8+ T cells leads to the development of memory-subsets with distinct homing and effector capacities. On non-lymphoid homing subsets, expression of "inflammatory" chemokine receptors (such as CXCR3, CCR5, CX3CR1 and CXCR1) is believed to promote migration into sites of infection/inflammation. Here we show that CXCR1 can be upregulated to the cell-surface within minutes of activating human CD8+ T cells. No concurrent upregulation of other inflammatory chemokine receptors was observed. Upregulation of CXCR1 preferentially occurred on central memory CD8+ T cells - i.e. cells with a lymph node homing phenotype - and was functionally relevant. Immunofluorescence microscopy showed CXCR1 to be present in intracellular vesicles that do not significantly colocalize with perforin, RANTES or the lysosomal marker CD63. By contrast, partial colocalization with the Golgi marker GM130, the constitutive secretory pathway marker 2 microglobulin and the early endosome marker EEA1 was observed. Upregulation of CXCR1 did not occur after T cell-receptor cross-linking. By contrast, supernatants from activated neutrophils, but not from monocytes or dendritic cells, induced its upregulation. These results suggest that CD8+ T cells can rapidly adapt their homing properties by mobilizing CXCR1 from a distinct intracellular compartment. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. Tseng-Rogenski and M. Liebert Interleukin-8 is essential for normal urothelial cell survival Am J Physiol Renal Physiol, September 1, 2009; 297(3): F816 - F821. [Abstract] [Full Text] [PDF] T. N. Hartmann, V. Grabovsky, R. Pasvolsky, Z. Shulman, E. C. Buss, A. Spiegel, A. Nagler, T. Lapidot, M. Thelen, and R. Alon A crosstalk between intracellular CXCR7 and CXCR4 involved in rapid CXCL12-triggered integrin activation but not in chemokine-triggered motility of human T lymphocytes and CD34+ cells J. Leukoc. Biol., October 1, 2008; 84(4): 1130 - 1140. [Abstract] [Full Text] [PDF] M. Hsu, S.-Y. Wu, S.-S. Chang, I.-J. Su, C.-H. Tsai, S.-J. Lai, A.-L. Shiau, K. Takada, and Y. Chang Epstein-Barr Virus Lytic Transactivator Zta Enhances Chemotactic Activity through Induction of Interleukin-8 in Nasopharyngeal Carcinoma Cells J. Virol., April 1, 2008; 82(7): 3679 - 3688. [Abstract] [Full Text] [PDF] L. Zheng, C.-n. Njauw, and M. Martins-Green A hCXCR1 transgenic mouse model containing a conditional color-switching system for imaging of hCXCL8/IL-8 functions in vivo J. Leukoc. Biol., November 1, 2007; 82(5): 1247 - 1256. [Abstract] [Full Text] [PDF] L. Zheng and M. Martins-Green Molecular mechanisms of thrombin-induced interleukin-8 (IL-8/CXCL8) expression in THP-1-derived and primary human macrophages J. Leukoc. Biol., September 1, 2007; 82(3): 619 - 629. [Abstract] [Full Text] [PDF] M. Jurewicz, D. H. McDermott, J. M. Sechler, K. Tinckam, A. Takakura, C. B. Carpenter, E. Milford, and R. Abdi Human T and Natural Killer Cells Possess a Functional Renin-Angiotensin System: Further Mechanisms of Angiotensin II-Induced Inflammation J. Am. Soc. Nephrol., April 1, 2007; 18(4): 1093 - 1102. [Abstract] [Full Text] [PDF] O. Gasser, T. A. Schmid, G. Zenhaeusern, and C. Hess Cyclooxygenase Regulates Cell Surface Expression of CXCR3/1-Storing Granules in Human CD4+ T Cells J. Immunol., December 15, 2006; 177(12): 8806 - 8812. [Abstract] [Full Text] [PDF]

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