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Blood, 1 October 2005, Vol. 106, No. 7, pp. 2347-2355. Prepublished online as a Blood First Edition Paper on June 28, 2005; DOI 10.1182/blood-2005-04-1407. This Article Full Text (PDF) All Versions of this Article: 2005-04-1407v1 106/7/2347    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Elsheikh, E. Articles by Sumitran-Holgersson, S. Search for Related Content PubMed PubMed Citation Articles by Elsheikh, E. Articles by Sumitran-Holgersson, S. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted April 6, 2005 Accepted June 4, 2005 Only a specific subset of human peripheral blood monocytes has endothelial-like functional capacity Elzafir Elsheikh, Mehmet Uzunel, Zhong He, Jan Holgersson, Grzegorz Nowak, and Suchitra Sumitran-Holgersson* Division of Transplantation Surgery, Karolinska Institutet, Karolinska University Hospital-Huddinge, Stockholm, Sweden Division of Clinical Immunology, Karolinska Institutet, Karolinska University Hospital-Huddinge, Stockholm, Sweden * Corresponding author; email: suchitra.holgersson{at}cfss.ki.se. The monocyte population in blood is considered as a possible source of endothelial precursors. Since endothelial-specific receptor tyrosine kinases act as regulators of endothelial cell functions, we investigated whether the expression of the vascular endothelial growth factor receptor-2 (VEGFR-2) on monocytes is important for their endothelial-like functional capacity. Peripheral blood monocytes expressing VEGFR-2 (CD14+/VEGFR-2+) were isolated and their phenotypic, morphological and functional capacity was compared with monocytes negative for this marker (CD14+/VEGFR-2-). CD14+/VEGFR-2+ cells constituted approximately 2.0±0.5% of the total population of monocytes and 0.08±0.04% of mononuclear cells in blood. CD14+/VEGFR-2+ cells exhibited the potential to differentiate in vitro into cells with endothelial characteristics. The cells were efficiently transduced by a lentiviral vector driving the expression of the green fluorescence protein (GFP). Transplantation of GFP-transduced cells into balloon-injured femoral arteries of nude mice significantly contributed to efficient re-endothelialization. CD14+/VEGFR-2- did not exhibit any of the above characteristics. These data demonstrate that expression of VEGFR-2 on peripheral blood monocytes is essential for their endothelial-like functional capacity, and supports the notion of a common precursor for monocytic and endothelial cell lineage. Our results help in clarifying which specific subpopulations may restore damaged endothelium and participate in the maintenance of vascular homeostasis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Circulating endothelial precursors: identification of functional subpopulations Stefanie Dimmeler Blood 2005 106: 2231-2232. [Full Text] [PDF] This article has been cited by other articles: H. Abou-Saleh, D. Yacoub, J.-F. Theoret, M.-A. Gillis, P.-E. Neagoe, B. Labarthe, P. 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