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Blood, 15 November 2005, Vol. 106, No. 10, pp. 3538-3545. Prepublished online as a Blood First Edition Paper on July 21, 2005; DOI 10.1182/blood-2005-04-1438. This Article Full Text (PDF) All Versions of this Article: 2005-04-1438v1 106/10/3538    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Jalili, A. Articles by Matsumoto, T. Search for Related Content PubMed PubMed Citation Articles by Jalili, A. Articles by Matsumoto, T. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted April 8, 2005 Accepted July 7, 2005 Induction of HM1.24 peptide-specific cytotoxic T lymphocytes by using peripheral blood stem cell harvests in patients with multiple myeloma Ali Jalili, Shuji Ozaki*, Tomoko Hara, Hironobu Shibata, Toshihiro Hashimoto, Masahiro Abe, Yasuhiko Nishioka, and Toshio Matsumoto Department of Medicine and Bioregulatory Sciences, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan Department of Medicine and Bioregulatory Sciences, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan; Division of Transfusion Medicine, Tokushima University Hospital, Tokushima, Japan Department of Internal Medicine and Molecular Therapeutics, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan * Corresponding author; email: ozakishu{at}clin.med.tokushima-u.ac.jp. HM1.24 antigen is preferentially overexpressed in multiple myeloma (MM) cells but not in normal cells. To explore the potential of HM1.24 as a target for cellular immunotherapy, we selected four HM1.24-derived peptides that possess binding motifs for HLA-A2 or HLA-A24 by using two computer-based algorithms. The ability of these peptides to generate cytotoxic T-lymphocytes (CTLs) was examined in 20 normal donors and 6 patients with MM by a reverse immunological approach. Dendritic cells (DCs) were induced from peripheral blood mononuclear cells of normal donors or peripheral blood stem cell (PBSC) harvests of MM patients, and autologous CD8+ T cells were stimulated with HM1.24 peptide-pulsed DCs. Both interferon--producing and cytotoxic responses were observed after stimulation with either HM1.24-126 or HM1.24-165 peptides in HLA-A2 and/or HLA-A24 individuals. The peptide-specific recognition of these CTLs was further confirmed by tetramer assay and cold target inhibition assay. Importantly, HM1.24-specific CTLs were also induced from PBSC harvests of MM patients and these CTLs were able to kill MM cells in an HLA-restricted manner. These results indicate the existence of functional DCs and HM1.24-specific CTL precursors within PBSC harvests, and provide the basis for cellular immunotherapy in combination with autologous PBSC transplantation in MM. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: E. Sekimoto, S. Ozaki, T. Ohshima, H. Shibata, T. Hashimoto, M. Abe, N. Kimura, K. Hattori, S. Kawai, Y. Kinoshita, et al. A Single-Chain Fv Diabody against Human Leukocyte Antigen-A Molecules Specifically Induces Myeloma Cell Death in the Bone Marrow Environment Cancer Res., February 1, 2007; 67(3): 1184 - 1192. [Abstract] [Full Text] [PDF]

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