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Blood, 15 October 2005, Vol. 106, No. 8, pp. 2871-2878. Prepublished online as a Blood First Edition Paper on July 5, 2005; DOI 10.1182/blood-2005-04-1522. This Article Full Text (PDF) All Versions of this Article: 2005-04-1522v1 106/8/2871    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Martin, P. Articles by Simon, M. M Search for Related Content PubMed PubMed Citation Articles by Martin, P. Articles by Simon, M. M Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted April 14, 2005 Accepted June 10, 2005 Quiescent and activated mouse granulocytes do not express granzyme A and B or perforin: similarities or differences with human polymorphonuclear leukocytes? Praxedis Martin, Reinhard Wallich, Julian Pardo, Arno Mullbacher, Markus Munder, Manuel Modelell, and Markus M Simon* Metschnikoff, Max-Planck-Institut fur Immunbiologie, Freiburg, Germany Institut fur Immunologie, Universitatsklinikum Heidelberg, Heidelberg, Germany John Curtin School of Medical Research, Australian National University, Canberra, Australia Medizinische Klinik 5, Universitat Heidelberg, Heidelberg, Germany * Corresponding author; email: simon{at}immunbio.mpg.de. Polymorphonuclear leukocytes have been shown to employ a multitude of effector functions to combat pathogens and tumors, including enzymes, defensins, and toxic products such as oxygen radicals and nitrogen oxides. Recent studies provided evidence for the expression of granzymes (gzm) and perforin (perf) within the cytotoxic arsenal of human neutrophils, the validity of which was questioned by two subsequent studies. We have now employed cytology, intracellular flow cytometry, enzymatic assays, immunoelectron microscopy and quantitative reverse transcriptase-polymerase chain reaction to obtain evidence of the presence of gzms and/or perf in mouse Gr-1+ granulocyte populations. The data obtained clearly demonstrate that neither in vitro nor in vivo-derived mouse granulocytes synthesize gzmA and gzmB or perf, even following infection/immunization with pathogens or pathogen-derived material. A parallel comparable analysis on the expression of gzmB in human neutrophils from three normal controls and four patients with diverse diseases failed to detect gzmB expression. The data indicate that polymorphonuclear leukocytes from mouse and man lack the three cytotoxic effector molecules, gzmA, gzmB and perf, generally associated with natural killer and cytotoxic T lymphocytes. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. M. Tschopp, N. Spiegl, S. Didichenko, W. Lutmann, P. Julius, J. C. Virchow, C. E. Hack, and C. A. Dahinden Granzyme B, a novel mediator of allergic inflammation: its induction and release in blood basophils and human asthma Blood, October 1, 2006; 108(7): 2290 - 2299. [Abstract] [Full Text] [PDF]

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