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Blood, 1 October 2005, Vol. 106, No. 7, pp. 2334-2339. Prepublished online as a Blood First Edition Paper on June 14, 2005; DOI 10.1182/blood-2005-04-1530. This Article Full Text (PDF) All Versions of this Article: 2005-04-1530v1 106/7/2334    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Dole, V. S Articles by Wagner, D. D Search for Related Content PubMed PubMed Citation Articles by Dole, V. S Articles by Wagner, D. D Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted April 15, 2005 Accepted May 31, 2005 Activated platelets induce Weibel-Palade body secretion and leukocyte rolling in vivo: role of P-selectin Vandana S Dole, Wolfgang Bergmeier, Heather A Mitchell, Sarah C Eichenberger, and Denisa D Wagner* CBR Institute for Biomedical Research, Inc., Boston, MA, USA; Department of Pathology, Harvard Medical School, Boston, MA, USA CBR Institute for Biomedical Research, Inc., Boston, MA, USA * Corresponding author; email: wagner{at}cbr.med.harvard.edu. The presence of activated platelets and platelet-leukocyte aggregates in circulation accompanies major surgical procedures and occurs in several chronic diseases. Recent findings that activated platelets contribute to the inflammatory disease atherosclerosis made us address the question whether activated platelets stimulate normal healthy endothelium. Infusion of activated platelets into young mice led to the formation of transient platelet-leukocyte aggregates and resulted in a several-fold systemic increase in leukocyte rolling 2-4 hours after infusion. Rolling returned to baseline levels 7 hours post-infusion. Infusion of activated P-selectin-/- platelets did not induce leukocyte rolling indicating that platelet P-selectin was involved in the endothelial activation. The endothelial activation did not require platelet CD40L. Leukocyte rolling was mediated solely by the interaction of endothelial P-selectin and leukocyte P-selectin glycoprotein ligand 1 (PSGL-1). Endothelial P-selectin is stored with von Willebrand factor (VWF) in Weibel-Palade bodies. The release of Weibel-Palade bodies upon infusion of activated platelets was indicated by both elevation of plasma VWF and by an increase in in vivo staining of endothelial P-selectin. We conclude that the presence of activated platelets in circulation promotes acute inflammation by stimulating secretion of Weibel-Palade bodies and P-selectin-mediated leukocyte rolling. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Platelets trigger endothelial exocytosis Charles J. Lowenstein Blood 2005 106: 2223. [Full Text] [PDF] This article has been cited by other articles: Yang Gu, Chang Liu, J. S. Alexander, L. J. Groome, and Yuping Wang Chymotrypsin-Like Protease (Chymase) Mediates Endothelial Activation by Factors Derived From Preeclamptic Placentas Reproductive Sciences, September 1, 2009; 16(9): 905 - 913. [Abstract] [PDF] T. E. Walshe, V. S. Dole, A. S.R. Maharaj, I. S. Patten, D. D. Wagner, and P. A. D'Amore Inhibition of VEGF or TGF-{beta} Signaling Activates Endothelium and Increases Leukocyte Rolling Arterioscler Thromb Vasc Biol, August 1, 2009; 29(8): 1185 - 1192. 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