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Blood, 1 November 2005, Vol. 106, No. 9, pp. 2982-2991. Prepublished online as a Blood First Edition Paper on August 2, 2005; DOI 10.1182/blood-2005-04-1543. This Article Full Text (PDF) Supplemental Figures All Versions of this Article: 2005-04-1543v1 106/9/2982    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sternberg, A. Articles by Vyas, P. Search for Related Content PubMed PubMed Citation Articles by Sternberg, A. Articles by Vyas, P. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted April 15, 2005 Accepted June 27, 2005 Evidence for reduced B-cell progenitors in early (low risk) myelodysplastic syndrome Alexander Sternberg, Sally Killick, Timothy Littlewood, Chris Hatton, Andrew Peniket, Thomas Seidl, Shammit Soneji, Joanne Leach, David Bowen, Claire Chapman, Edwin Massey, Graham Standen, Lisa Robinson, Bipin Vadher, Richard Kaczmarski, Riaz Janmohammed, Kim Clipsham, Andrew Carr, and Paresh Vyas* MRC Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, Oxford, United Kingdom; John Radcliffe Hospital, Oxford, United Kingdom Royal Bournemouth Hospital, Bournemouth, United Kingdom John Radcliffe Hospital, Oxford, United Kingdom Institute of Cancer Research, London, United Kingdom MRC Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, Oxford, United Kingdom Ninewells Hospital, Dundee, United Kingdom Leicester Royal Infirmary, Leicester, United Kingdom Bristol Royal Infirmary, Bristol, United Kingdom Hereford County Hospital, Hereford, United Kingdom Princess Alexandra Hospital, Farnborough, United Kingdom Hillingdon Hospital, London, United Kingdom Nuffield Orthopaedic Centre, Oxford, United Kingdom * Corresponding author; email: paresh.vyas{at}imm.ox.ac.uk. Early, low risk IPSS (International Prognostic Scoring System) myelodysplasia (MDS) is a heterogeneous disorder where the molecular and cellular haematopoietic defects are poorly understood. To gain insight into this condition, we analyzed gene expression profiles of marrow CD34+ progenitor cells from normal karyotype, low blast count MDS patients, age-matched controls and patients with non-MDS anaemia. Given the heterogeneity of early MDS, a surprisingly consistent finding was decreased expression of B-cell lineage affiliated genes in MDS patients compared to normal controls and 3/5 samples with non-MDS anaemia. Both cases of non-MDS anaemia with reduced B-cell gene expression were on chemotherapy. In 25/27 of the original samples and 9 further MDS samples, Taqman Real Time PCR confirmed these data. Flow cytometry on unfractionated marrow from independent samples also demonstrated reduced B-cell progenitors in MDS patients compared to normal controls. These novel findings suggest a common perturbation in early MDS haematopoiesis. They also provide the rationale for a larger study to evaluate the diagnostic utility of reduced B-cell progenitor number as a diagnostic biomarker of early low risk MDS which can pose a diagnostic challenge. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. L. Chen, T. D. Logan, M. L. Hochberg, S. G. Shelat, X. Yu, G. E. Wilding, W. Tan, G. C. Kujoth, T. A. Prolla, M. A. Selak, et al. Erythroid dysplasia, megaloblastic anemia, and impaired lymphopoiesis arising from mitochondrial dysfunction Blood, November 5, 2009; 114(19): 4045 - 4053. [Abstract] [Full Text] [PDF] L. Sutton, M. Vusirikala, and W. Chen Hematogone Hyperplasia in Copper Deficiency Am J Clin Pathol, August 1, 2009; 132(2): 191 - 199. [Abstract] [Full Text] [PDF] N. Goardon, E. Nikolousis, A. Sternberg, W.-K. Chu, C. Craddock, P. Richardson, R. Benson, M. 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Ossenkoppele Identification of distinct prognostic subgroups in low- and intermediate-1-risk myelodysplastic syndromes by flow cytometry Blood, February 1, 2008; 111(3): 1067 - 1077. [Abstract] [Full Text] [PDF] K. Ogata, Y. Kishikawa, C. Satoh, H. Tamura, K. Dan, and A. Hayashi Diagnostic application of flow cytometric characteristics of CD34+ cells in low-grade myelodysplastic syndromes Blood, August 1, 2006; 108(3): 1037 - 1044. [Abstract] [Full Text] [PDF] A. Pellagatti, M. Cazzola, A. A. N. Giagounidis, L. Malcovati, M. G. D. Porta, S. Killick, L. J. Campbell, L. Wang, C. F. Langford, C. Fidler, et al. Gene expression profiles of CD34+ cells in myelodysplastic syndromes: involvement of interferon-stimulated genes and correlation to FAB subtype and karyotype Blood, July 1, 2006; 108(1): 337 - 345. [Abstract] [Full Text] [PDF]

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