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Blood, 1 February 2006, Vol. 107, No. 3, pp. 1141-1148. Prepublished online as a Blood First Edition Paper on October 4, 2005; DOI 10.1182/blood-2005-04-1722. This Article Full Text (PDF) All Versions of this Article: 2005-04-1722v1 107/3/1141    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lenze, D. Articles by Hummel, M. Search for Related Content PubMed PubMed Citation Articles by Lenze, D. Articles by Hummel, M. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted April 28, 2005 Accepted September 23, 2005 Influence of antigen on the development of MALT-lymphoma Dido Lenze, Erika Berg, Rudolf Volkmer-Engert, Armin A Weiser, Axel Greiner, Constanze Knorr-Wittmann, Ioannis Anagnostopoulos, Harald Stein, and Michael Hummel* Institute of Pathology, Campus Benjamin Franklin, Charite-Universitatsmedizin Berlin, Berlin, Germany Institute of Medical Immunology, Campus Mitte, Charite-Universitatsmedizin Berlin, Berlin, Germany Institute of Pathology, University of Wurzburg, Wurzburg, Germany * Corresponding author; email: michael.hummel{at}charite.de. Mucosa Associated Lymphoid Tissue (MALT) B-cell lymphomas develop in the context of autoimmune or chronic inflammations like Helicobacter pylori induced gastritis. Remission of most gastric MALT-lymphomas after eradication of Helicobacter pylori links tumour cell proliferation to antigen-induced inflammation and the need for antigenic contact. Furthermore the tumour cells correspond to antigen-activated memory B-cells. To investigate the reactivity of the tumour immunoglobulins we employed in-vitro generated antibodies identical with those produced by MALT-lymphoma cells. The immunoglobulin rearrangements of seven MALT-lymphomas were amplified, cloned and expressed as single chain fragment variable (scFv) antibodies. Antigen specificity of these seven scFvs was analyzed by immunohistochemical staining of various normal, reactive and malignant human tissues. Also an expression library comprising approximately 30.000 proteins from human fetal brain (protein filter) and a peptide library were screened. One scFv stained a subpopulation of tonsillar plasma cells in immunohistochemical studies. On protein filters this scFv recognized the plasma cell related protein Ufc1. Peptide library screening identified nine peptides as binding partners of a further scFv. The majority of MALT-lymphoma immunoglobulins studied however showed no reactivity against antigens indicating that the tumor immunoglobulins do not play a significant role in stimulation and proliferation of the MALT-lymphoma tumor cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: F. van Maldegem, R. van Dijk, T. A. M. Wormhoudt, P. M. Kluin, R. Willemze, L. Cerroni, C. J. M. van Noesel, and R. J. Bende The majority of cutaneous marginal zone B-cell lymphomas expresses class-switched immunoglobulins and develops in a T-helper type 2 inflammatory environment Blood, October 15, 2008; 112(8): 3355 - 3361. [Abstract] [Full Text] [PDF] U. Hershberg, M. Uduman, M. J. Shlomchik, and S. H. Kleinstein Improved methods for detecting selection by mutation analysis of Ig V region sequences Int. Immunol., May 1, 2008; 20(5): 683 - 694. [Abstract] [Full Text] [PDF]

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