Blood online
Home About Blood Authors Subscriptions Permission Advertising Public Access contact us
 

 
Advanced
Current Issue
First Edition
Future Articles
Archives
Submit to Blood
Search
American Society of Hematology
Meeting Abstracts
Email Alerts
 Blood, 1 April 2006, Vol. 107, No. 7, pp. 2879-2881.
Prepublished online as a Blood First Edition Paper on December 1, 2005; DOI 10.1182/blood-2005-05-1815.

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
2005-05-1815v1
107/7/2879    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Right arrow Rights and Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hoebeke, I.
Right arrow Articles by Leclercq, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hoebeke, I.
Right arrow Articles by Leclercq, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

arrow to previous article Previous Article  |  Next Article next article arrow

Submitted May 4, 2005
Accepted November 20, 2005

Overexpression of HES-1 is not sufficient to impose T-cell differentiation on human hematopoietic stem cells

Inge Hoebeke, Magda De Smedt, Inge Van de Walle, Katia Reynvoet, Greet De Smet, Jean Plum, and Georges Leclercq*

Department of Clinical Chemistry, Microbiology and Immunology, Faculty of Medicine and Health Sciences, Ghent University, Ghent University Hospital, Ghent, Belgium

* Corresponding author; email: georges.leclercq{at}ugent.be.

By retroviral overexpression of the Notch-1 intracellular domain (ICN) in human CD34+ HSCs, we have shown previously that Notch-1 signalling promotes the T-cell fate and inhibits the monocyte and B-cell fate in several in vitro and in vivo differentiation assays. Here, we investigated whether the effects of constitutively active Notch-1 can be mimicked by overexpression of its downstream target gene HES-1. Upon HES-1 retroviral transduction, human CD34+ stem cells had a different outcome in the differentiation assays as compared to ICN-transduced cells. Although HES-1 induced a partial block in B-cell development, it did not inhibit monocyte development and did not promote T/NK-cell lineage differentiation. On the contrary, a higher percentage of HES-1-transduced stem cells remained CD34+. These experiments indicate that HES-1 alone is not able to substitute for Notch-1 signalling to induce T-cell differentiation of human CD34+ hematopoietic stem cells.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
E. Kleinmann, A.-S. Geimer Le Lay, M. Sellars, P. Kastner, and S. Chan
Ikaros Represses the Transcriptional Response to Notch Signaling in T-Cell Development
Mol. Cell. Biol., December 15, 2008; 28(24): 7465 - 7475.
[Abstract] [Full Text] [PDF]


click for free articles
home about blood authors subscriptions permissions advertising public access contact us
  Copyright © 2005 by American Society of Hematology         Online ISSN: 1528-0020