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Blood, 1 November 2005, Vol. 106, No. 9, pp. 3268-3270. Prepublished online as a Blood First Edition Paper on July 19, 2005; DOI 10.1182/blood-2005-05-1873. This Article Full Text (PDF) All Versions of this Article: 2005-05-1873v1 106/9/3268    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kemna, E. Articles by Swinkels, D. Search for Related Content PubMed PubMed Citation Articles by Kemna, E. Articles by Swinkels, D. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted May 9, 2005 Accepted June 6, 2005 Novel urine hepcidin assay by mass spectrometry Erwin Kemna*, Harold Tjalsma, Coby Laarakkers, Elizabeta Nemeth, Hans Willems, and Dorine Swinkels Department of Clinical Chemistry, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA, USA * Corresponding author; email: E.Kemna{at}akc.umcn.nl. Hepatic peptide hormone hepcidin is the central regulator of iron metabolism and mediator of anemia of inflammation. To date, only one specific immuno-dot assay to measure hepcidin in urine had been documented. Here we report an alternative approach for quantification of hepcidin in urine by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS). Peptide peaks were detected corresponding to the three forms of hepcidin normally found in urine. The identity of the peptide peak equivalent to hepcidin-25 was confirmed using synthetic human hepcidin-25. Validation of our MS data on samples with various hepcidin levels showed a strong correlation with previous immuno-dot assay results (Spearman R = 0.9275, P< 0.0001). Most importantly, this hepcidin assay clearly discriminates between relevant clinical iron disorders. In conclusion, this novel MS urine hepcidin assay is easy to perform and available to a wide audience. This enables the implementation of hepcidin measurements in large clinical studies. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: H. P.E. Peters, C. M.M. Laarakkers, D. W. Swinkels, and J. F.M. Wetzels Serum hepcidin-25 levels in patients with chronic kidney disease are independent of glomerular filtration rate Nephrol. Dial. Transplant., October 23, 2009; (2009) gfp546v1. [Abstract] [Full Text] [PDF] J. Malyszko Hepcidin Assays: Ironing Out Some Details Clin. J. Am. Soc. Nephrol., June 1, 2009; 4(6): 1015 - 1016. [Full Text] [PDF] S. A. Browne and D. Reddan Potential role of bone morphogenetic protein (BMP) signalling as a potential therapeutic target for modification of iron balance Nephrol. Dial. Transplant., January 1, 2009; 24(1): 28 - 30. [Full Text] [PDF] C. Brugnara An immunoassay for human serum hepcidin at last: Ganz klar? Blood, November 15, 2008; 112(10): 3922 - 3923. [Full Text] [PDF] T. Ganz, G. Olbina, D. Girelli, E. Nemeth, and M. Westerman Immunoassay for human serum hepcidin Blood, November 15, 2008; 112(10): 4292 - 4297. [Abstract] [Full Text] [PDF] J. F. Collins, M. Wessling-Resnick, and M. D. Knutson Hepcidin Regulation of Iron Transport J. Nutr., November 1, 2008; 138(11): 2284 - 2288. [Abstract] [Full Text] [PDF] D. W. Swinkels and J. F. M. Wetzels Hepcidin: a new tool in the management of anaemia in patients with chronic kidney disease? Nephrol. Dial. Transplant., August 1, 2008; 23(8): 2450 - 2453. [Full Text] [PDF] E. H.J.M. Kemna, H. Tjalsma, H. L. Willems, and D. W. Swinkels Hepcidin: from discovery to differential diagnosis Haematologica, January 1, 2008; 93(1): 90 - 97. [Abstract] [Full Text] [PDF] C. T. Howard, U. S. McKakpo, I. A. Quakyi, K. M. Bosompem, E. A. Addison, K. Sun, D. Sullivan, and R. D. Semba Relationship of Hepcidin with Parasitemia and Anemia among Patients with Uncomplicated Plasmodium falciparum Malaria in Ghana Am J Trop Med Hyg, October 1, 2007; 77(4): 623 - 626. [Abstract] [Full Text] [PDF] K. J. A. Vanhoutte, C. Laarakkers, E. Marchiori, P. Pickkers, J. F. M. Wetzels, J. L. Willems, L. P. van den Heuvel, F. G. M. Russel, and R. Masereeuw Biomarker discovery with SELDI-TOF MS in human urine associated with early renal injury: evaluation with computational analytical tools Nephrol. Dial. Transplant., October 1, 2007; 22(10): 2932 - 2943. [Abstract] [Full Text] [PDF] J. l. Sullivan Macrophage Iron, Hepcidin, and Atherosclerotic Plaque Stability Experimental Biology and Medicine, September 1, 2007; 232(8): 1014 - 1020. [Abstract] [Full Text] [PDF] A. T. Murphy, D. R. Witcher, P. Luan, and V. J. Wroblewski Quantitation of hepcidin from human and mouse serum using liquid chromatography tandem mass spectrometry Blood, August 1, 2007; 110(3): 1048 - 1054. [Abstract] [Full Text] [PDF] E. H.J.M. Kemna, H. Tjalsma, V. N. Podust, and D. W. Swinkels Mass Spectrometry-Based Hepcidin Measurements in Serum and Urine: Analytical Aspects and Clinical Implications Clin. Chem., April 1, 2007; 53(4): 620 - 628. [Abstract] [Full Text] [PDF] T. Ganz Molecular Control of Iron Transport J. Am. Soc. Nephrol., February 1, 2007; 18(2): 394 - 400. [Abstract] [Full Text] [PDF] N. Tomosugi, H. Kawabata, R. Wakatabe, M. Higuchi, H. Yamaya, H. Umehara, and I. Ishikawa Detection of serum hepcidin in renal failure and inflammation by using ProteinChip System Blood, August 15, 2006; 108(4): 1381 - 1387. [Abstract] [Full Text] [PDF] D. W. Swinkels, M. C.H. Janssen, J. Bergmans, and J. J.M. Marx Hereditary Hemochromatosis: Genetic Complexity and New Diagnostic Approaches Clin. Chem., June 1, 2006; 52(6): 950 - 968. [Abstract] [Full Text] [PDF]

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