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Blood, 15 November 2005, Vol. 106, No. 10, pp. 3374-3376. Prepublished online as a Blood First Edition Paper on August 4, 2005; DOI 10.1182/blood-2005-05-1889. This Article Full Text (PDF) Supplemental Table All Versions of this Article: 2005-05-1889v1 106/10/3374    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kralovics, R. Articles by Skoda, R. C Search for Related Content PubMed PubMed Citation Articles by Kralovics, R. Articles by Skoda, R. C Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted May 10, 2005 Accepted July 11, 2005 Altered gene expression in myeloproliferative disorders correlates with activation of signaling by the V617F mutation of Jak2 Robert Kralovics, Soon-Siong Teo, Andreas S Buser, Martin Brutsche, Ralph Tiedt, Andre Tichelli, Francesco Passamonti, Daniela Pietra, Mario Cazzola, and Radek C Skoda* Experimental Hematology, Department of Research, University Hospital Basel, Basel, Switzerland Division of Clinical Hematology, University Hospital Basel, Basel, Switzerland Division of Pneumology, University Hospital Basel, Basel, Switzerland Division of Diagnostic Hematology, University Hospital Basel, Basel, Switzerland Division of Hematology, University of Pavia Medical School and IRCCS Policlinico S. Matteo, Pavia, Italy * Corresponding author; email: radek.skoda{at}unibas.ch. We identified 13 new gene expression markers that were elevated and one marker, ANKRD15, that was downregulated in patients with polycythemia vera (PV). These 14 markers, as well as the previously described PRV1 and NF-E2, exhibited the same gene expression alterations also in patients with exogenously activated granulocytes due to sepsis or G-CSF treatment. The recently described V617F mutation in the Janus kinase 2 (JAK2) gene allows defining subclasses of patients with myeloproliferative disorders based on the JAK2 genotype. PV patients homozygous or heterozygous for JAK2-V617F exhibited higher levels of expression of the 13 new markers, PRV1 and NF-E2 than patients without JAK2-V617F, whereas ANKRD15 was downregulated in these patients. Our results suggest that the alterations in expression of the markers studied are due to the activation of the Jak/STAT pathway through exogenous stimuli (sepsis or G-CSF treatment), or endogenously through the JAK2-V617F mutation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: V617F "JAKs" up myeloproliferative signal Ayalew Tefferi Blood 2005 106: 3335-3336. [Full Text] [PDF] This article has been cited by other articles: L. Pieri, C. Bogani, P. Guglielmelli, M. Zingariello, R. A. Rana, N. Bartalucci, A. Bosi, and A. M. Vannucchi The JAK2V617 mutation induces constitutive activation and agonist hypersensitivity in basophils from patients with polycythemia vera Haematologica, November 1, 2009; 94(11): 1537 - 1545. [Abstract] [Full Text] [PDF] N. Fourouclas, J. Li, D. C. Gilby, P. J. Campbell, P. A. Beer, E. M. Boyd, A. C. Goodeve, D. Bareford, C. N. Harrison, J. T. Reilly, et al. Methylation of the suppressor of cytokine signaling 3 gene (SOCS3) in myeloproliferative disorders Haematologica, November 1, 2008; 93(11): 1635 - 1644. [Abstract] [Full Text] [PDF] K. Liu, R. Kralovics, Z. Rudzki, B. Grabowska, A. S. Buser, D. Olcaydu, H. 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[Full Text] [PDF] E. Lippert, M. Boissinot, R. Kralovics, F. Girodon, I. Dobo, V. Praloran, N. Boiret-Dupre, R. C. Skoda, and S. Hermouet The JAK2-V617F mutation is frequently present at diagnosis in patients with essential thrombocythemia and polycythemia vera Blood, September 15, 2006; 108(6): 1865 - 1867. [Abstract] [Full Text] [PDF] C. Bellanne-Chantelot, I. Chaumarel, M. Labopin, F. Bellanger, V. Barbu, C. De Toma, F. Delhommeau, N. Casadevall, W. Vainchenker, G. Thomas, et al. Genetic and clinical implications of the Val617Phe JAK2 mutation in 72 families with myeloproliferative disorders Blood, July 1, 2006; 108(1): 346 - 352. [Abstract] [Full Text] [PDF] A. I. Schafer Molecular basis of the diagnosis and treatment of polycythemia vera and essential thrombocythemia Blood, June 1, 2006; 107(11): 4214 - 4222. [Abstract] [Full Text] [PDF] F. Passamonti, E. Rumi, D. Pietra, M. G. D. Porta, E. Boveri, C. Pascutto, L. Vanelli, L. Arcaini, S. Burcheri, L. Malcovati, et al. 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