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Blood, 1 March 2006, Vol. 107, No. 5, pp. 2180-2183. Prepublished online as a Blood First Edition Paper on November 8, 2005; DOI 10.1182/blood-2005-05-1922. This Article Full Text (PDF) Supplemental Table and Figures All Versions of this Article: 2005-05-1922v1 107/5/2180    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Narayan, A D Articles by Zanjani, E. D Search for Related Content PubMed PubMed Citation Articles by Narayan, A D Articles by Zanjani, E. D Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted May 11, 2005 Accepted October 21, 2005 Human embryonic stem cell-derived hematopoietic cells are capable of engrafting primary as well as secondary fetal sheep recipients A D Narayan*, Jessica L Chase, Rachel L Lewis, Xinghui Tian, Dan S Kaufman, James A Thomson, and Esmail D Zanjani Department of Animal Biotechnology, University of Nevada, Reno, NV, USA Wisconsin Regional Primate Research Center, University of Wisconsin, Madison, WI, USA Stem Cell Institute and Department of Medicine, Division of Hematology, Oncology, and Transplantation, University of Minnesota, Minneapolis, MN, USA Wisconsin Regional Primate Research Center, University of Wisconsin, Madison, WI, USA; Department of Anatomy, School of Medicine, University of Wisconsin, Madison, WI, USA * Corresponding author; email: daisy{at}med.unr.edu. The human/sheep xenograft model has proven valuable in assessing the in vivo hematopoietic activity of stem cells from a variety of fetal and post-natal human sources. CD34+/Lineage- or CD34+/CD38- cells isolated from human embryonic stem (hES) cells differentiated on S17 feeder layer were transplanted by intraperitoneal injections into fetal sheep. Chimerism in primary transplants was established with PCR and flowcytometry of bone marrow and peripheral blood samples. Whole bone marrow cells harvested from a primary recipient were transplanted into a secondary recipient. Chimerism was established as before. This animal was challenged with human GM-CSF and an increase in human hematopoietic activity was noted by flowcytometry. Bone marrow aspirations cultured in methylcellulose generated colonies identified to be of human origin by PCR. We therefore conclude that hES cells are capable of generating hematopoietic cells that engraft primary recipients. These cells also fulfill the criteria for long-term engrafting hematopoietic stem cells as demonstrated by engraftment and differentiation in the secondary recipient. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. S. Kaufman Toward clinical therapies using hematopoietic cells derived from human pluripotent stem cells Blood, October 22, 2009; 114(17): 3513 - 3523. [Abstract] [Full Text] [PDF] Z. Su, C. Frye, K.-M. Bae, V. Kelley, and J. Vieweg Differentiation of Human Embryonic Stem Cells into Immunostimulatory Dendritic Cells under Feeder-Free Culture Conditions Clin. Cancer Res., October 1, 2008; 14(19): 6207 - 6217. [Abstract] [Full Text] [PDF]

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