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Blood, 1 December 2005, Vol. 106, No. 12, pp. 3926-3931. Prepublished online as a Blood First Edition Paper on August 9, 2005; DOI 10.1182/blood-2005-05-1972. This Article Full Text (PDF) All Versions of this Article: 2005-05-1972v1 106/12/3926    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Zambello, R. Articles by Semenzato, G. Search for Related Content PubMed PubMed Citation Articles by Zambello, R. Articles by Semenzato, G. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted May 17, 2005 Accepted July 28, 2005 Phenotypic and functional analyses of dendritic cells in patients with lymphoproliferative disease of granular lymphocytes (LDGL) Renato Zambello, Tamara Berno, Giovanna Cannas, Ilenia Baesso, Gianni Binotto, Emanuela Bonoldi, Pierantonio Bevilacqua, Marta Miorin, Monica Facco, Livio Trentin, Carlo Agostini, and Gianpietro Semenzato* Department of Clinical and Experimental Medicine, Hematology and Clinical Immunology Branch, Padua University School of Medicine, University of Padua, Padova, Italy; Venetian Institute of Molecular Medicine (VIMM), Padova, Italy; Istituto Oncologico Veneto (IOV), Padova, Italy Pathology Department, San Bortolo Hospital, Vicenza, Italy * Corresponding author; email: g.semenzato{at}unipd.it. We investigated whether dendritic cells (DC) play a role in favouring granular lymphocytes (GL) proliferation in LDGL patients. The presence of in vivo circulating DC was studied in 11 patients (5 CD3+ and 6 CD3- LDGL). Autologous immature (iDC) and mature (mDC) DC generated in vitro were studied for stimulatory activity on cell proliferation of CD3+ and CD3- GL. The topographic organization of GL and DC was also studied in bone marrow (BM) biopsies. PB CD3- GL from patients showed significant proliferative activity in the presence of iDC and mDC. Conversely, monoclonal CD3+ GL were unresponsive to autologous and allogeneic PB DC. Analysis of BM biopsies demonstrated a topographic distribution of DC and GL that indicates contact between the two cell types. On functional assays, DC obtained from BM were more efficient than PB DC in stimulating CD3- GL, and surprisingly, a low but definite stimulatory effect was demonstrated also on CD3+ GL. The putative contact between DC and GL in the BM and, more crucial, the proliferative response of discrete GL populations to DC stimulation suggest the presence of a specific antigen within BM DC, providing evidence for a role of DC in the pathogenesis of LDGL. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: R. Zambello and G. Semenzato Large granular lymphocyte disorders: new etiopathogenetic clues as a rationale for innovative therapeutic approaches Haematologica, October 1, 2009; 94(10): 1341 - 1345. [Full Text] [PDF]

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