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Blood, 15 November 2005, Vol. 106, No. 10, pp. 3490-3497. Prepublished online as a Blood First Edition Paper on July 19, 2005; DOI 10.1182/blood-2005-05-2044. This Article Full Text (PDF) All Versions of this Article: 2005-05-2044v1 106/10/3490    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Penna, G. Articles by Adorini, L. Search for Related Content PubMed PubMed Citation Articles by Penna, G. Articles by Adorini, L. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted May 20, 2005 Accepted July 12, 2005 Expression of the inhibitory receptor ILT3 on dendritic cells is dispensable for induction of CD4+Foxp3+ regulatory T cells by 1,25-dihydroxyvitamin D3 Giuseppe Penna, Andrea Roncari, Susana Amuchastegui, Kenn C Daniel, Emilio Berti, Marco Colonna, and Luciano Adorini* BioXell, Milano, Italy Department of Dermatology, University of Milano-Bicocca and Ospedale Maggiore-IRCCS, Milano, Italy Department of Pathology and Immunology, Washinghton University School of Medicine, St. Louis, MO, USA * Corresponding author; email: luciano.adorini{at}bioxell.com. 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] is a secosteroid hormone that renders dendritic cells (DCs) tolerogenic, favoring the induction of regulatory T cells. Induction of DCs with tolerogenic properties by 1,25(OH)2D)3 is associated with increased selective expression of immunoglobulin-like transcript (ILT)3, suggesting its involvement in the immunoregulatory properties of this hormone. Here we show an in vivo correlate of the increased ILT3 expression on DCs in healing psoriatic lesions following topical treatment with the 1,25(OH))2D)3 analog calcipotriol. Analysis of DC subsets reveals a differential regulation of ILT3 expression by 1,25(OH))2D)3, with a marked upregulation in myeloid DCs but no effect on its expression by plasmacytoid DCs. A regulatory role for ILT3 expressed on DCs is indicated by the increased IFN- secretion promoted by anti-ILT3 addition to cultures of DCs and T cells but this effect is blunted in 1,25(OH))2D)3-treated DCs, suggesting ILT3-independent mechanisms able to regulate T cell activation. Although ILT3 expression by DCs is required for induction of regulatory T cells, DC pretreatment with 1,25(OH))2D)3 leads to induction of CD4+Foxp3+ cells with suppressive activity irrespective of the presence of neutralizing anti-ILT3 mAb, indicating that ILT3 expression is dispensable for the capacity of 1,25(OH))2D)3-treated DCs to induce regulatory T cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Savy, K. Edmond, P. E. M. Fine, A. Hall, B. J. Hennig, S. E. Moore, K. Mulholland, U. Schaible, and A. M. Prentice Landscape Analysis of Interactions between Nutrition and Vaccine Responses in Children J. Nutr., November 1, 2009; 139(11): 2154S - 2218S. [Abstract] [Full Text] [PDF] S. Chamorro, J. J. Garcia-Vallejo, W. W. J. Unger, R. J. Fernandes, S. C. M. Bruijns, S. Laban, B. O. Roep, B. A. 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