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Blood, 15 April 2006, Vol. 107, No. 8, pp. 3197-3204. Prepublished online as a Blood First Edition Paper on December 27, 2005; DOI 10.1182/blood-2005-05-2185. This Article Full Text (PDF) All Versions of this Article: 2005-05-2185v1 107/8/3197    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ohmori, K. Articles by Kannagi, R. Search for Related Content PubMed PubMed Citation Articles by Ohmori, K. Articles by Kannagi, R. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted May 31, 2005 Accepted November 28, 2005 Identification of cutaneous lymphocyte-associated antigen as sialyl 6-sulfo Lewis X, a selectin ligand expressed on a subset of skin-homing helper memory T cells Katsuyuki Ohmori, Fumiyo Fukui, Makoto Kiso, Toshio Imai, Osamu Yoshie, Hitoshi Hasegawa, Kouji Matsushima, and Reiji Kannagi* Department of Clinical Pathology, Kyoto University School of Medicine, Kyoto, Japan Department of Molecular Pathology, Aichi Cancer Center Research Institute, Nagoya, Aichi, Japan Department of Applied Bio-organic Chemistry, Faculty of Agriculture, Gifu University, Gifu, Japan Department of Bacteriology, Kinki University School of Medicine, Osaka, Japan First Department of Internal Medicine, Ehime University School of Medicine, Ehime, Japan Department of Molecular Preventive Medicine, School of Medicine, University of Tokyo, Tokyo, Japan Department of Molecular Pathology, Aichi Cancer Center Research Institute, Nagoya, Aichi, Japan; CREST, Japan Science and Technology Agency, Kawaguchi, Saitama, Japan * Corresponding author; email: rkannagi{at}aichi-cc.jp. We previously identified the carbohydrate determinant, sialyl 6-sulfo Lex, as the major L-selectin ligand on high endothelial venules of peripheral lymph nodes. In this study we studied distribution of the sialyl 6-sulfo Lex determinant among peripheral lymphocytes. The determinant was expressed on a subset of helper memory T-and NK cells. The helper memory T-cells expressing sialyl 6-sulfo Lex were CD45RObright+PSGL-1high+CCR4+L-selectin+CCR7+, but did not express 47 integrin or CCR9, indicating that they were the skin-homing population of central memory T-cells. The T-cell subset significantly expressed mRNA for 6-sulfotransferase HEC-GlcNAc6ST and fucosyltransferase Fuc-T VII responsible for the synthesis of sialyl 6-sulfo Lexx The characteristics of the T-cell population were similar to those previously described for cutaneous lymphocyte-associated antigen (CLA)-positive T cells defined by the HECA-452 or 2F3 antibody. The binding of the T-cell subset with the specific anti-sialyl 6-sulfo Lex antibody, G152, was almost completely abrogated by HECA-452 or 2F3. Binding of recombinant E-, P- and L-selectins to the T cell subset was significantly inhibited by G152 as well as HECA-452 antibodies. We propose that CLA, which is expressed without any activation stimuli on peripheral skin-homing helper memory T cells in normal individuals, is at least partly the sialyl 6-sulfo Lex determinant. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. C. Kerr, C. B. Fieger, K. R. Snapp, and S. D. Rosen Endoglycan, a Member of the CD34 Family of Sialomucins, Is a Ligand for the Vascular Selectins J. Immunol., July 15, 2008; 181(2): 1480 - 1490. [Abstract] [Full Text] [PDF] E. E. Eriksson No detectable endothelial- or leukocyte-derived L-selectin ligand activity on the endothelium in inflamed cremaster muscle venules J. Leukoc. Biol., July 1, 2008; 84(1): 93 - 103. [Abstract] [Full Text] [PDF] M. E. Gainers, L. Descheny, S. R. Barthel, L. Liu, M.-A. Wurbel, and C. J. Dimitroff Skin-Homing Receptors on Effector Leukocytes Are Differentially Sensitive to Glyco-Metabolic Antagonism in Allergic Contact Dermatitis J. Immunol., December 15, 2007; 179(12): 8509 - 8518. [Abstract] [Full Text] [PDF] N. Kimura, K. Ohmori, K. Miyazaki, M. Izawa, Y. Matsuzaki, Y. Yasuda, H. Takematsu, Y. Kozutsumi, A. Moriyama, and R. Kannagi Human B-lymphocytes Express {alpha}2-6-Sialylated 6-Sulfo-N-acetyllactosamine Serving as a Preferred Ligand for CD22/Siglec-2 J. Biol. Chem., November 2, 2007; 282(44): 32200 - 32207. [Abstract] [Full Text] [PDF] S. Julien, M. J. Grimshaw, M. Sutton-Smith, J. Coleman, H. R. Morris, A. Dell, J. Taylor-Papadimitriou, and J. M. Burchell Sialyl-Lewisx on P-Selectin Glycoprotein Ligand-1 Is Regulated during Differentiation and Maturation of Dendritic Cells: A Mechanism Involving the Glycosyltransferases C2GnT1 and ST3Gal I J. Immunol., November 1, 2007; 179(9): 5701 - 5710. [Abstract] [Full Text] [PDF] G.-Y. Chen, H. Osada, L. F. Santamaria-Babi, and R. Kannagi Interaction of GATA-3/T-bet transcription factors regulates expression of sialyl Lewis X homing receptors on Th1/Th2 lymphocytes PNAS, November 7, 2006; 103(45): 16894 - 16899. [Abstract] [Full Text] [PDF]

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