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Blood, 15 January 2006, Vol. 107, No. 2, pp. 602-609. Prepublished online as a Blood First Edition Paper on September 8, 2005; DOI 10.1182/blood-2005-06-2234. This Article Full Text (PDF) Supplemental Tables and Figures All Versions of this Article: 2005-06-2234v1 107/2/602    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Anichini, A. Articles by Di Nicola, M. Search for Related Content PubMed PubMed Citation Articles by Anichini, A. Articles by Di Nicola, M. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted June 3, 2005 Accepted August 11, 2005 Skewed T cell differentiation in indolent non-Hodgkin's lymphoma patients reversed by ex-vivo T cell culture with c-cytokines Andrea Anichini, Roberta Mortarini, Luca Romagnoli, Paola Baldassari, Antonello Cabras, Carmelo Carlo-Stella, Alessandro M Gianni*, and Massimo Di Nicola Human Tumor Immunobiology Unit, Department of Experimental Oncology, Istituto Nazionale per lo Studio e la Cura dei Tumori, Milan, Italy "Cristina Gandini" Bone Marrow Transplantation Unit, Department of Medical Oncology, Istituto Nazionale per lo Studio e la Cura dei Tumori, Milan, Italy Department of Pathology, Istituto Nazionale per lo Studio e la Cura dei Tumori, Milan, Italy "Cristina Gandini" Bone Marrow Transplantation Unit, Department of Medical Oncology, Istituto Nazionale per lo Studio e la Cura dei Tumori, Milan, Italy; Chair of Medical Oncology, University of Milan, Milan, Italy * Corresponding author; email: alessandro.gianni{at}unimi.it. The unfavourable clinical evolution in indolent non-Hodgkin's lymphomas suggests defective control of neoplastic growth by the immune system. To address this issue, we evaluated phenotype, function and maturation profile of CD4+ and CD8+ T cells from peripheral blood, lymph nodes or bone marrow of B cell NHL patients at diagnosis. T cells from these patients frequently showed an activated, but apoptosis-prone phenotype with low frequency of tumor-reactive T cells showing a TH2/Tc2 functional profile in the response to autologous tumor. In peripheral blood or in lymph nodes and bone marrow, and in comparison to healthy donors, patients' T cells showed a skewed differentiation towards Tnaive and TCentral Memory stages, with low expression of granzyme B and perforin. T cell culture with autologous tumor in the presence of IL-2, IL-15 and autologous bone-marrow-derived cells, led to massive T cell expansion and to differentiation of cytotoxic factor+ CD8+ T cells releasing IFN- and killing autologous B cell tumor in an HLA-Class I-restricted fashion. These results suggest impaired T cell differentiation to effector stage in B cell NHL patients, but indicate that T cell responsiveness to c cytokines is retained, thus allowing to promote generation of anti-tumor T cells for immune intervention. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. Anichini, R. Mortarini, D. Nonaka, A. Molla, C. Vegetti, E. Montaldi, X. Wang, and S. Ferrone Association of Antigen-Processing Machinery and HLA Antigen Phenotype of Melanoma Cells with Survival in American Joint Committee on Cancer Stage III and IV Melanoma Patients. Cancer Res., June 15, 2006; 66(12): 6405 - 6411. [Abstract] [Full Text] [PDF]

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