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Blood, 1 January 2006, Vol. 107, No. 1, pp. 213-221.
Prepublished online as a Blood First Edition Paper on September 13, 2005; DOI 10.1182/blood-2005-06-2273.
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Submitted June 7, 2005
Accepted August 11, 2005
Transmembrane adaptor molecules: A new category of lymphoid cell markers
Sara Tedoldi, Jennifer C Paterson, Martin-Leo Hansmann, Yasodha Natkunam, Thomas Ruediger, Pavla Angelisova, Ming Q Du, Helen Roberton, Giovanna Roncador, Lydia Sanchez, Michela Pozzobon, Noraidah Masir, Richard Barry, Stefano Pileri, David Y Mason, Teresa Marafioti*, and Vaclav Horejsi
Leukaemia Research Fund Immunodiagnostics Unit, Nuffield Department of Clinical Laboratory Sciences, John Radcliffe Hospital, Oxford, United Kingdom
Senckenberg Pathology Institute, Johann Wolfgang Goethe-University Clinic Frankfurt am Main, Frankfurt am Main, Germany
Department of Pathology, Stanford University School of Medicine, Stanford, California, USA
Institute of Pathology, University of Wuerzburg, Wuerzburg, Germany
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic
Division of Molecular Histopathology, Department of Pathology, University of Cambridge, Addenbrookes Hospital, Cambridge, United Kingdom
Monoclonal Antibodies Unit, Biotechnology Program, National Center for the Investigation of Oncology, Madrid, Spain
Department of of Pathology, Unit of Haematopathology, "L&A Seragnoli" Institute of Haematology, University of Bologna, Bologna, Italy
* Corresponding author; email: teresa.marafioti{at}ndcls.ox.ac.uk.
Transmembrane adaptor proteins (of which seven have been identified so far) are involved in receptor signaling in immune cells. They have only a short extracellular region, most of the molecule comprising a substantial intracytoplasmic region carrying multiple tyrosine residues that can be phosphorylated by Src- or Syk-family kinases. In this paper we report an immunohistologic study of six of these molecules in normal and neoplastic human tissue sections, and show that they are restricted to sub-populations of lymphoid cells, being present in either T cells (LAT, LIME and TRIM), B cells (NTAL), or subsets of both cell types (PAG and SIT). Their expression in neoplastic lymphoid cells broadly reflects that of normal lymphoid tissue, including the positivity of plasma cells and myeloma/plasmacytoma for LIME, NTAL, PAG and SIT. However, this study also revealed some reactions that may be of diagnostic/prognostic value. For example, lymphocytic lymphoma and mantle cell lymphoma showed similar profiles but differed clearly from follicle center lymphoma, while PAG tended to be selectively expressed in germinal center-derived subsets of diffuse large B cell lymphoma. These molecules represent a potentially important addition to the panel of immunophenotypic markers detectable in routine biopsies that can be used in hematopathologic studies.
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