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Blood, 1 April 2006, Vol. 107, No. 7, pp. 2830-2838. Prepublished online as a Blood First Edition Paper on December 6, 2005; DOI 10.1182/blood-2005-06-2403. This Article Full Text (PDF) All Versions of this Article: 2005-06-2403v1 107/7/2830    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Seddiki, N. Articles by Fazekas de St Groth, B. Search for Related Content PubMed PubMed Citation Articles by Seddiki, N. Articles by Fazekas de St Groth, B. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted June 16, 2005 Accepted November 21, 2005 Persistence of naive CD45RA+ regulatory T cells in adult life Nabila Seddiki, Brigitte Santner-Nanan, Stuart G Tangye, Stephen I Alexander, Michael Solomon, Soon Lee, Ralph Nanan, and Barbara Fazekas de St Groth* Centenary Institute of Cancer Medicine and Cell Biology, Newtown, NSW, Australia Department of Pediatrics, University of Sydney, Western Clinical School, Penrith, NSW, Australia The Childrens Hospital at Westmead, Westmead, NSW, Australia The Royal Prince Alfred Hospital, Camperdown, NSW, Australia * Corresponding author; email: b.fazekas{at}centenary.usyd.edu.au. Regulatory T cells (Treg) constitutively expressing CD4, CD25 and the transcription factor Foxp3 can prevent a wide range of experimental and spontaneous autoimmune diseases in mice. In humans, CD4+CD25bright T cells, predominantly within the CD45RO+ activated/memory subset in adults and the CD45RA+ naive T cell subset in infants, are considered to be the equivalent subset. Using novel combinations of mAbs, we examined expression of CD25 in human infant thymus, cord blood, adult peripheral blood, lymph node and spleen. In addition to the CD4+CD25bright T cells, subfractionation on the basis of CD45 splice variants indicated that all samples contained a second distinct population of cells expressing a slightly lower level of CD25. In adult peripheral blood, this population expressed a naive CD45RA+ phenotype. The corresponding population in lymph node, spleen and cord blood showed some evidence of activation, and expressed markers characteristic of Treg, such as CTLA-4. Sorted CD4+CD25+CD45RA+ T cells from both cord and adult blood expressed very high levels of mRNA for Foxp3 and manifested equivalent suppressive activity in vitro, indicating that they are bone fide members of the regulatory T cell lineage. Targeting naive Treg in adults may offer new means of preventing and treating autoimmune disease. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: H. Grindebacke, H. Stenstad, M. Quiding-Jarbrink, J. Waldenstrom, I. Adlerberth, A. E. Wold, and A. Rudin Dynamic Development of Homing Receptor Expression and Memory Cell Differentiation of Infant CD4+CD25high Regulatory T Cells J. Immunol., October 1, 2009; 183(7): 4360 - 4370. [Abstract] [Full Text] [PDF] J. J. Zaunders, M. L. Munier, N. Seddiki, S. Pett, S. Ip, M. Bailey, Y. Xu, K. Brown, W. B. Dyer, M. Kim, et al. 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