SEARCH:   Advanced

Blood, 15 February 2006, Vol. 107, No. 4, pp. 1636-1642. Prepublished online as a Blood First Edition Paper on November 1, 2005; DOI 10.1182/blood-2005-06-2559. This Article Full Text (PDF) All Versions of this Article: 2005-06-2559v1 107/4/1636    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wang, R. Articles by Chandawarkar, R. Y Search for Related Content PubMed PubMed Citation Articles by Wang, R. Articles by Chandawarkar, R. Y Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted June 29, 2005 Accepted October 12, 2005 Exogenous Heat Shock Protein-70 binds macrophage-lipid raft-microdomain and stimulates phagocytosis, processing and MHC-II presentation of antigens Ruibo Wang, Joseph T Kovalchin, Peggy Muhlenkamp, and Rajiv Y Chandawarkar* Division of Plastic Surgery, Department of Surgery, Center for Immunotherapy, University of Connecticut School of Medicine, Farmington, CT, USA * Corresponding author; email: chandawarkar{at}uchc.edu. The extracellular presence of endotoxin-free Heat Shock Protein 70 (HSP70) enhances the rate and capacity of macrophage-mediated phagocytosis at 6 times the basal rate. It is protein-specific, dose- and time-dependent and involves the internalization of inert microspheres, gram-positive and -negative bacteria and fungi. Structurally, exogenous HSP70 binds the macrophage plasma membrane, specifically on its lipid raft-microdomain. Disruption of lipid rafts, HSP70-LR interaction or denaturing HSP70 abrogates the HSP-mediated increase in phagocytosis. Further, HSP70-mediated phagocytosis directly enhances the processing and presentation of internalized antigens via the endocytic MHC class-II pathway to CD4+ T lymphocytes. Modulating the HSP70-LR interaction presents an opportunity to intervene at the level of host pathogen interface; a therapeutic tool for emerging infections, especially where conventional treatment with antibiotics is ineffective (antibiotic resistance) or unavailable (rapidly spreading). These results identify a new role for HSP70, a highly conserved molecule in stimulating phagocytosis - a primordial macrophage function thereby influencing both innate and adaptive immune responses. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: H. Shiratsuchi, Y. Kouatli, G. X. Yu, H. M. Marsh, and M. D. Basson Propofol inhibits pressure-stimulated macrophage phagocytosis via the GABAA receptor and dysregulation of p130cas phosphorylation Am J Physiol Cell Physiol, June 1, 2009; 296(6): C1400 - C1410. [Abstract] [Full Text] [PDF] A. Briolay, J. Bouzenzana, M. Guichardant, C. Deshayes, N. Sindt, L. Bessueille, and V. Bulone Cell Wall Polysaccharide Synthases Are Located in Detergent-Resistant Membrane Microdomains in Oomycetes Appl. Envir. Microbiol., April 1, 2009; 75(7): 1938 - 1949. [Abstract] [Full Text] [PDF] V. L. Vega, M. Rodriguez-Silva, T. Frey, M. Gehrmann, J. C. Diaz, C. Steinem, G. Multhoff, N. Arispe, and A. De Maio Hsp70 Translocates into the Plasma Membrane after Stress and Is Released into the Extracellular Environment in a Membrane-Associated Form that Activates Macrophages J. Immunol., March 15, 2008; 180(6): 4299 - 4307. [Abstract] [Full Text] [PDF] A. Osterloh, A. Veit, A. Gessner, B. Fleischer, and M. Breloer Hsp60-mediated T cell stimulation is independent of TLR4 and IL-12 Int. Immunol., March 1, 2008; 20(3): 433 - 443. [Abstract] [Full Text] [PDF] H. Bendz, S. C. Ruhland, M. J. Pandya, O. Hainzl, S. Riegelsberger, C. Brauchle, M. P. Mayer, J. Buchner, R. D. Issels, and E. Noessner Human Heat Shock Protein 70 Enhances Tumor Antigen Presentation through Complex Formation and Intracellular Antigen Delivery without Innate Immune Signaling J. Biol. Chem., October 26, 2007; 282(43): 31688 - 31702. [Abstract] [Full Text] [PDF] A. Katz, P. Waridel, A. Shevchenko, and U. Pick Salt-induced Changes in the Plasma Membrane Proteome of the Halotolerant Alga Dunaliella salina as Revealed by Blue Native Gel Electrophoresis and Nano-LC-MS/MS Analysis Mol. Cell. Proteomics, September 1, 2007; 6(9): 1459 - 1472. [Abstract] [Full Text] [PDF] L. K. Marriott, K. R. McGann-Gramling, B. Hauss-Wegrzyniak, L. C. Sheldahl, R. A. Shapiro, D. M. Dorsa, and G. L. Wenk Brain Infusion of Lipopolysaccharide Increases Uterine Growth as a Function of Estrogen Replacement Regimen: Suppression of Uterine Estrogen Receptor-{alpha} by Constant, But Not Pulsed, Estrogen Replacement Endocrinology, January 1, 2007; 148(1): 232 - 240. [Abstract] [Full Text] [PDF]

	Copyright © 2005 by American Society of Hematology         Online ISSN: 1528-0020