SEARCH:   Advanced

Blood, 1 April 2006, Vol. 107, No. 7, pp. 2904-2911. Prepublished online as a Blood First Edition Paper on December 8, 2005; DOI 10.1182/blood-2005-07-2615. This Article Full Text (PDF) Supplemental Table All Versions of this Article: 2005-07-2615v1 107/7/2904    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Buhl, A. M. Articles by Leffers, H. Search for Related Content PubMed PubMed Citation Articles by Buhl, A. M. Articles by Leffers, H. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted July 1, 2005 Accepted November 15, 2005 Identification of a gene on chromosome 12q22 uniquely overexpressed in chronic lymphocytic leukemia Anne Mette Buhl*, Jesper Jurlander, Flemming S Jorgensen, Anne Marie Ottesen, Jack B Cowland, Lise Mette Gjerdrum, Brian V Hansen, and Henrik Leffers The Leukemia Laboratory, Department of Hematology, Rigshospitalet, Copenhagen, Denmark Department of Medicinal Chemistry, Danish University of Pharmaceutical Sciences, Copenhagen, Denmark University Department of Growth and Reproduction, Rigshospitalet, Copenhagen, Denmark The Granulocyte Laboratory, Department of Hematology, Rigshospitalet, Copenhagen, Denmark Department of Pathology, Rigshospitalet, Copenhagen, Denmark * Corresponding author; email: am.buhl{at}get2net.dk. The pathogenesis of chronic lymphocytic leukemia (CLL) is unknown, but may involve aberrant activation of signaling pathways. Somatic hypermutations in rearranged immunoglobulin heavy chain (IgVH) genes allow a division of CLL patients into two categories; mutated IgVH genes are associated with an indolent disease, whereas unmutated IgVH genes define an aggressive form. Using differential display to compare gene expression in CLL cells with and without IgVH hypermutations, we identified a novel gene, CLL Upregulated gene1 (CLLU1) that was highly upregulated in CLL cells without IgVH hypermutations. CLLU1 mapped to chromosome 12q22, within a cluster of genes that are active in germinal center B-cells. However, appreciable levels of CLLU1 were only detectable in CLL cells, and not in a panel of normal tissue extracts, nor in any other tested hematological malignancy. High expression of CLLU1 in CLL samples occurred irrespective of trisomy 12 or large chromosomal rearrangements. CLLU1 encodes six mRNAs with no sequence homology to any known gene, and most transcripts appear to be non-coding. Two transcripts, however, potentially encode a peptide with remarkable structural similarity to human IL-4. These data, in particular the unique and restricted expression pattern, suggest that CLLU1 is the first disease specific gene identified in CLL. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: A novel gene for an old disease Federico Caligaris-Cappio Blood 2006 107: 2594. [Full Text] [PDF] This article has been cited by other articles: A. Siepel Darwinian alchemy: Human genes from noncoding DNA Genome Res., October 1, 2009; 19(10): 1693 - 1695. [Full Text] [PDF] D. G. Knowles and A. McLysaght Recent de novo origin of human protein-coding genes Genome Res., October 1, 2009; 19(10): 1752 - 1759. [Abstract] [Full Text] [PDF] P. Josefsson, C. H. Geisler, H. Leffers, J. H. Petersen, M. K. Andersen, J. Jurlander, and A. M. Buhl CLLU1 expression analysis adds prognostic information to risk prediction in chronic lymphocytic leukemia Blood, June 1, 2007; 109(11): 4973 - 4979. [Abstract] [Full Text] [PDF]

	Copyright © 2005 by American Society of Hematology         Online ISSN: 1528-0020