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Blood, 1 February 2006, Vol. 107, No. 3, pp. 980-986. Prepublished online as a Blood First Edition Paper on September 29, 2005; DOI 10.1182/blood-2005-07-2733. This Article Full Text (PDF) All Versions of this Article: 2005-07-2733v1 107/3/980    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Huang, X. Articles by Lind, S. E Search for Related Content PubMed PubMed Citation Articles by Huang, X. Articles by Lind, S. E Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted July 11, 2005 Accepted September 23, 2005 A soluble tissue factor-annexin v chimeric protein has both procoagulant and anticoagulant properties Xin Huang, Wei-Qun Ding, Joshua L Vaught, Roman F Wolf, James H Morrissey, Roger G Harrison, and Stuart E Lind* Departments of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA Dept. of Biochemistry and College of Medicine, University of Illinois at Urbana-Champaign, Urbana, IL, USA School of Chemical, Biological and Materials Engineering, University of Oklahoma, Norman, OK, USA Departments of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA; Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA * Corresponding author; email: stuart-lind{at}ouhsc.edu. Tissue factor (TF) initiates blood coagulation, but its expression in the vascular space requires a finite period of time. We hypothesized that targeting exogenous tissue factor to sites of vascular injury could lead to accelerated hemostasis. Since phosphatidylserine (PS) is exposed on activated cells at sites of vascular injury, we cloned the cDNA for a chimeric protein consisting of the extracellular domain of TF (called soluble TF or sTF) and annexin V, a human PS-binding protein. Both the sTF and annexin V domains had ligand-binding activities consistent with their native counterparts, and the chimera accelerated Factor X activation by Factor VIIa. The chimera exhibited bi-phasic effects upon blood coagulation. At low concentrations it accelerated blood coagulation, while at higher concentrations it acted as an anticoagulant . The chimera accelerated coagulation in the presence of either unfractionated or low molecular weight heparins more potently than Factor VIIa, and shortened the bleeding time of mice treated with enoxaparin. The sTF-annexin V chimera is a targeted procoagulant protein that may be useful in accelerating thrombin generation where PS is exposed to the vasculature, such as may occur at sites of vascular injury or on within the vasculature of tumors. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Portable tissue factor to the rescue Gary E. Gilbert Blood 2006 107: 856-857. [Full Text] [PDF]

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