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Blood, 1 July 2006, Vol. 108, No. 1, pp. 262-269. Prepublished online as a Blood First Edition Paper on March 7, 2006; DOI 10.1182/blood-2005-07-2878. This Article Full Text (PDF) All Versions of this Article: 2005-07-2878v1 108/1/262    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Maeda, K. Articles by Ogawa, H. Search for Related Content PubMed PubMed Citation Articles by Maeda, K. Articles by Ogawa, H. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted July 20, 2005 Accepted February 23, 2006 FOG-1 represses GATA-1-dependent FcRI -chain transcription: transcriptional mechanism of mast cell-specific gene expression in mice Keiko Maeda, Chiharu Nishiyama*, Tomoko Tokura, Hiroyasu Nakano, Shunsuke Kanada, Makoto Nishiyama, Ko Okumura, and Hideoki Ogawa Atopy Research Center, Juntendo University School of Medicine, Bunkyo-ku, Tokyo, Japan Department of Immunology, Juntendo University School of Medicine, Bunkyo-ku, Tokyo, Japan Atopy Research Center, Juntendo University School of Medicine, Bunkyo-ku, Tokyo, Japan; Department of Immunology, Juntendo University School of Medicine, Bunkyo-ku, Tokyo, Japan Biotechnology Research Center, The University of Tokyo, Bunkyo-ku, Tokyo, Japan * Corresponding author; email: chinishi{at}med.juntendo.ac.jp. Cell-type specific transcription of mouse high-affinity IgE receptor (FcRI) -chain is positively regulated by the transcription factor, GATA-1. Although GATA-1 is expressed in erythroid cells, megakaryocytes, and mast cells, the expression of mouse FcRI -chain is restricted to mast cells. In the present study, we characterized the role of GATA-associated cofactor FOG-1 in the regulation of the FcRI -chain promoter. The expression levels of FOG-1, GATA-1, and -chain in each hematopoietic cell line were analyzed by RT-PCR and western blotting. FOG-1 expression was higher in the -chain negative hematopoietic progenitor cell line, Ba/F3, than in the -chain positive mast cell line, PT18. By contrast, GATA-1 expression was similar when comparing the two cell lines. A transient reporter assay demonstrated that the -chain promoter functioned in PT18 but not in Ba/F3 and that the transcription activity of the -chain promoter in PT18 was markedly suppressed by overexpression of FOG-1. Although the activity of the -chain promoter, which was up-regulated by co-expression of GATA-1, was significantly suppressed by co-expression of FOG-1 in the simian kidney CV-1 cells [-chain-, GATA-1-, and FOG-1-), the transactivation of the -chain promoter by the GATA-1 mutant, V205G, which can not bind FOG-1, was not affected by co-expression of FOG-1. Further, overexpression of FOG-1 in PT18 resulted in decreases in cell surface expression of FcRI and -chain transcription. Finally, suppression of FOG-1 expression using a siRNA approach resulted in increased -chain promoter activity in Ba/F3. These results suggest FOG-1 that expression level regulates the GATA-1-dependent FcRI -chain promoter. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: R. K. Miller, H. Qadota, T. J. Stark, K. B. Mercer, T. S. Wortham, A. Anyanful, and G. M. 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Fedosyuk, R. Neades, and K. R. Peterson Silencing of A{gamma}-Globin Gene Expression during Adult Definitive Erythropoiesis Mediated by GATA-1-FOG-1-Mi2 Complex Binding at the -566 GATA Site Mol. Cell. Biol., May 15, 2008; 28(10): 3101 - 3113. [Abstract] [Full Text] [PDF] A. B. Cantor, H. Iwasaki, Y. Arinobu, T. B. Moran, H. Shigematsu, M. R. Sullivan, K. Akashi, and S. H. Orkin Antagonism of FOG-1 and GATA factors in fate choice for the mast cell lineage J. Exp. Med., March 17, 2008; 205(3): 611 - 624. [Abstract] [Full Text] [PDF] N. Nakano, C. Nishiyama, S. Kanada, Y. Niwa, N. Shimokawa, H. Ushio, M. Nishiyama, K. Okumura, and H. Ogawa Involvement of mast cells in IL-12/23 p40 production is essential for survival from polymicrobial infections Blood, June 1, 2007; 109(11): 4846 - 4855. [Abstract] [Full Text] [PDF] B. Ghinassi, M. Sanchez, F. Martelli, G. Amabile, A. M. Vannucchi, G. Migliaccio, S. H. Orkin, and A. R. 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