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Blood, 15 April 2006, Vol. 107, No. 8, pp. 3359-3370. Prepublished online as a Blood First Edition Paper on December 27, 2005; DOI 10.1182/blood-2005-07-2888. This Article Full Text (PDF) All Versions of this Article: 2005-07-2888v1 107/8/3359    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Hodge, D. Articles by Perkins, A. Search for Related Content PubMed PubMed Citation Articles by Hodge, D. Articles by Perkins, A. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted July 19, 2005 Accepted September 30, 2005 A global role for EKLF in definitive and primitive erythropoiesis Denise Hodge, Elise Coghill, Janelle Keys, Tina Maguire, Belinda Hartmann, Alasdair McDowall, Mitchell Weiss, Sean Grimmond, and Andrew Perkins* Institute for Molecular Biosciences, University of Queensland, Brisbane, QLD, Australia Institute for Molecular Biosciences, University of Queensland, Brisbane, QLD, Australia; Australian Special Research Centre for Functional and Applied Genomics, Philadelphia, PA, USA The Children's Hospital of Philadelphia and the University of Pennsylvania, Philadelphia, PA, USA * Corresponding author; email: a.perkins{at}imb.uq.edu.au. Erythroid Kruppel-like factor, (EKLF, KLF1), plays an important role in definitive erythropoiesis and -globin gene regulation 1,2, but failure to rectify lethal fetal anemia upon correction of globin chain imbalance suggested additional critical EKLF target genes 3. We employed expression profiling of EKLF null fetal liver and EKLF null erythroid cell lines containing an inducible EKLF-ERTM fusion construct to search for such targets. An overlapping list of EKLF-regulated genes from the two systems included -hemoglobin stabilizing protein (AHSP), cytoskeletal proteins, heme synthesis enzymes, transcription factors and blood group antigens. One EKLF target gene, dematin, which encodes an erythrocyte cytoskeletal protein (band 4.9), contains several phylogenetically conserved consensus CACC motifs predicted to bind EKLF. Chromatin immunoprecipitation demonstrated in vivo EKLF occupancy at these sites and promoter reporter assays showed that ELKF activates gene transcription through these DNA elements. Furthermore, investigation of EKLF target genes in the yolk sac lead to the discovery of unexpected additional defects in the embryonic red cell membrane and cytoskeleton. In short, EKLF regulates global erythroid gene expression which is critical for the development of primitive and definitive red cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. R. Tallack, J. R. Keys, P. O. Humbert, and A. C. Perkins EKLF/KLF1 Controls Cell Cycle Entry via Direct Regulation of E2f2 J. Biol. Chem., July 31, 2009; 284(31): 20966 - 20974. [Abstract] [Full Text] [PDF] T. Sengupta, N. Cohet, F. Morle, and J. J. 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