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Blood, 1 March 2006, Vol. 107, No. 5, pp. 2123-2130.
Prepublished online as a Blood First Edition Paper on November 8, 2005; DOI 10.1182/blood-2005-08-3099.
Previous Article | Next Article 
Submitted August 2, 2005
Accepted October 18, 2005
Annexin 1 and its bioactive peptide inhibit neutrophil-endothelium interactions under flow: indication of distinct receptor involvement
Richard P Hayhoe, Ahmad M Kamal, Egle Solito, Roderick J Flower, Dianne Cooper, and Mauro Perretti*
Centre for Biochemical Pharmacology, The William Harvey Research Institute, London, United Kingdom
Department of Cellular and Molecular Neuroscience, Faculty of Medicine, Imperial College London, Hammersmith Hospital Campus, London, United Kingdom
* Corresponding author; email: m.perretti{at}qmul.ac.uk.
We have tested the effects of annexin 1 (ANXA1) and its N-terminal peptide Ac2-26 on PMN recruitment under flow. Differential effects of the full-length protein and its peptide were observed; ANXA1 inhibited firm adhesion of human PMN, whilst Ac2-26 significantly attenuated capture and rolling without effect on firm adhesion. Analysis of the effects of ANXA1 and Ac2-26 on PMN adhesion molecule expression supported the flow chamber results, with Ac2-26 but not ANXA1 causing L-selectin and PSGL-1 shedding. ANXA1 and its peptide act via the FPR family of receptors. This was corroborated using HEK-293 cells transfected with FPR or FPRL-1/ALX (the two members of this family expressed by human PMN). While Ac2-26 bound both FPR and FPRL-1/ALX, ANXA1 bound FPRL-1/ALX only. ANXA1 and Ac2-26 acted as genuine agonists; Ac2-26 binding led to ERK activation in both FPR and FPRL-1/ALX transfected cells while ANXA1 caused ERK activation only in cells transfected with FPRL-1/ALX. Finally blockade of FPRL-1/ALX with a neutralising monoclonal antibody was found to abrogate the effects of ANXA1 in the flow chamber but was without effect on Ac2-26-mediated inhibition of rolling. These findings demonstrate for the first time distinct mechanisms of action for ANXA1 and its N-terminal peptide Ac2-26.

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