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Blood, 1 February 2006, Vol. 107, No. 3, pp. 1063-1069. Prepublished online as a Blood First Edition Paper on October 18, 2005; DOI 10.1182/blood-2005-08-3123. This Article Full Text (PDF) All Versions of this Article: 2005-08-3123v1 107/3/1063    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Liu, Z. Articles by Mayadas, T. N Search for Related Content PubMed PubMed Citation Articles by Liu, Z. Articles by Mayadas, T. N Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted August 3, 2005 Accepted September 26, 2005 Differential roles for 2 integrins in experimental autoimmune bullous pemphigoid Zhi Liu*, Minglang Zhao, Ning Li, Luis A Diaz, and Tanya N Mayadas Department of Dermatology, University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, NC, USA; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, NC, USA Department of Dermatology, University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, NC, USA Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA * Corresponding author; email: zhiliu{at}med.unc.edu. Bullous pemphigoid (BP) is an autoimmune disease associated with autoantibodies directed against the hemidesmosomal antigens anti-BP230 and anti-B180. Neonatal mice injected with rabbit anti-mouse BP180 IgG develop a BP-like disease. Complement, immune complexes, mast cells, and neutrophils play a key role in subepidermal blistering in this animal model. In this study we investigated the role of 2 integrins in experimental BP. Wild-type (WT) mice pretreated with neutralizing antibody against CD11a (LFA-1), CD11b (Mac-1), CD11a plus CD11b, or CD18 alone failed to develop BP when injected with pathogenic anti-mBP180 IgG. This was associated with a significant reduction in neutrophil accumulation in neutralizing antibody treated mice. Mac-1-deficient (Mac-1 KO) mice were resistant to experimental BP despite normal complement deposition and mast cell and neutrophil degranulation. Neutrophil infiltration in Mac-1 KO mice was severely impaired at 24 h. However, more neutrophils accumulated in the skin of Mac-1 KO mice compared to WT mice at early time points (2-4 h), which was associated with an increase in their survival as determined by apoptosis markers. These data suggest that 2 integrins play differential roles in experimental BP: LFA-1 is required for neutrophil recruitment while Mac-1 mediates late neutrophil accumulation and apoptosis of infiltrating neutrophils. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Beautiful blistering Clifford A. Lowell Blood 2006 107: 849-850. [Full Text] [PDF] This article has been cited by other articles: A. Sindrilaru, T. Peters, J. Schymeinsky, T. Oreshkova, H. Wang, A. Gompf, F. Mannella, M. Wlaschek, C. Sunderkotter, K. L. Rudolph, et al. Wound healing defect of Vav3-/- mice due to impaired {beta}2-integrin-dependent macrophage phagocytosis of apoptotic neutrophils Blood, May 21, 2009; 113(21): 5266 - 5276. [Abstract] [Full Text] [PDF] R. Paul, B. Obermaier, J. Van Ziffle, B. Angele, H.-W. Pfister, C. A. Lowell, and U. Koedel Myeloid Src kinases regulate phagocytosis and oxidative burst in pneumococcal meningitis by activating NADPH oxidase J. Leukoc. Biol., October 1, 2008; 84(4): 1141 - 1150. [Abstract] [Full Text] [PDF] M. Lauterbach, P. O'Donnell, K. Asano, and T. N. Mayadas Role of TNF priming and adhesion molecules in neutrophil recruitment to intravascular immune complexes J. Leukoc. Biol., June 1, 2008; 83(6): 1423 - 1430. [Abstract] [Full Text] [PDF] A. Utomo, J. Hirahashi, D. Mekala, K. Asano, M. Glogauer, X. Cullere, and T. N. Mayadas Requirement for Vav Proteins in Post-Recruitment Neutrophil Cytotoxicity in IgG but Not Complement C3-Dependent Injury J. Immunol., May 1, 2008; 180(9): 6279 - 6287. [Abstract] [Full Text] [PDF] S. U. Eisenhardt, M. Schwarz, N. Schallner, J. Soosairajah, N. Bassler, D. Huang, C. Bode, and K. Peter Generation of activation-specific human anti-{alpha}M{beta}2 single-chain antibodies as potential diagnostic tools and therapeutic agents Blood, April 15, 2007; 109(8): 3521 - 3528. [Abstract] [Full Text] [PDF] J. Y. Park, M. Amin Arnaout, and V. Gupta A Simple, No-Wash Cell Adhesion-Based High-Throughput Assay for the Discovery of Small-Molecule Regulators of the Integrin CD11b/CD18 J Biomol Screen, April 1, 2007; 12(3): 406 - 417. [Abstract] [PDF] Z. Li, A. R. Burns, and C. W. Smith Lymphocyte Function-Associated Antigen-1-Dependent Inhibition of Corneal Wound Healing Am. J. Pathol., November 1, 2006; 169(5): 1590 - 1600. [Abstract] [Full Text] [PDF]

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