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Blood, 15 March 2006, Vol. 107, No. 6, pp. 2400-2408. Prepublished online as a Blood First Edition Paper on November 17, 2005; DOI 10.1182/blood-2005-08-3340. This Article Full Text (PDF) Supplemental Table All Versions of this Article: 2005-08-3340v1 107/6/2400    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Xu, H. Articles by Spain, L. M Search for Related Content PubMed PubMed Citation Articles by Xu, H. Articles by Spain, L. M Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted August 17, 2005 Accepted October 28, 2005 Patterns of expression, membrane localization, and effects of ectopic expression suggest a function for MS4a4B, a CD20 homologue in Th1 T cells Hui Xu, Mark S Williams*, and Lisa M Spain Department of Microbiology and Immunology and Center for Vascular and Inflammatory Diseases, University of Maryland School of Medicine, Baltimore, MD, USA * Corresponding author; email: marwilliams{at}som.umaryland.edu. The membrane-spanning 4A, (MS4A) family of proteins includes CD20, FcRI and HTm4, whose genes are grouped in a chromosomal location that is associated with increased susceptibility to allergy and atopic asthma. One family member, Chandra/MS4a4B, was reported to be expressed in Th1 T cells, but not Th2 T cells. In the present study, MS4a4B was isolated in a screen of genes differentially expressed during thymocyte development. MS4a4B was detected in immature CD4-8-CD44+CD25- thymocytes, turned off during further stages of thymocyte development, and re-expressed in mature single-positive thymocytes. MS4a4B expression was found in naive CD8+ and CD4+ peripheral T cells and NK cells, but not in B cells. MS4a4B is expressed at the cell surface with its C-terminus located in the cytoplasm. When expressed in a T cell hybridoma by retroviral vector, MS4a4B protein constitutively associates with lipid raft microdomains while in primary T cells, endogenous MS4a4B protein becomes enriched in rafts after T cell activation. Overexpression of MS4a4B in primary CD4+ T cell blasts enhanced TCR-induced Th1 cytokine production. These results suggest that MS4a4B expression is tightly regulated during T cell development and that MS4a4B expression promotes Th1 function and/or differentiation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: Y. Xiao, V. Peperzak, A. M. Keller, and J. Borst CD27 Instructs CD4+ T Cells to Provide Help for the Memory CD8+ T Cell Response after Protein Immunization J. Immunol., July 15, 2008; 181(2): 1071 - 1082. [Abstract] [Full Text] [PDF] H. Xu, T. B. Oriss, M. Fei, A. C. Henry, B. N. Melgert, L. Chen, A. L. Mellor, D. H. Munn, C. G. Irvin, P. Ray, et al. Indoleamine 2,3-dioxygenase in lung dendritic cells promotes Th2 responses and allergic inflammation PNAS, May 6, 2008; 105(18): 6690 - 6695. [Abstract] [Full Text] [PDF] C. Coban, K. J. Ishii, S. Uematsu, N. Arisue, S. Sato, M. Yamamoto, T. Kawai, O. Takeuchi, H. Hisaeda, T. Horii, et al. Pathological role of Toll-like receptor signaling in cerebral malaria Int. Immunol., January 1, 2007; 19(1): 67 - 79. [Abstract] [Full Text] [PDF]

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