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Blood, 1 May 2006, Vol. 107, No. 9, pp. 3779-3786. Prepublished online as a Blood First Edition Paper on January 3, 2006; DOI 10.1182/blood-2005-08-3501. This Article Full Text (PDF) All Versions of this Article: 2005-08-3501v1 107/9/3779    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Brickner, A. G Articles by Warren, E. H Search for Related Content PubMed PubMed Citation Articles by Brickner, A. G Articles by Warren, E. H Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted August 30, 2005 Accepted December 20, 2005 The PANE1 gene encodes a novel human minor histocompatibility antigen that is selectively expressed in B-lymphoid cells and B-CLL Anthony G Brickner, Anne M Evans, Jeffrey K Mito, Suzanne M Xuereb, Xin Feng, Tetsuya Nishida, Liane Fairfull, Robert E Ferrell, Kenneth A Foon, Donald F Hunt, Jeffrey Shabanowitz, Victor H Engelhard, Stanley R Riddell, and Edus H Warren* University of Pittsburgh Cancer Institute and Departments of Medicine and Immunology, University of Pittsburgh School of Medicine, Piitsburgh, PA, USA Department of Microbiology and Carter Immunology Center, and Departments of Chemistry and Pathology, University of Virginia, Charlottesville, VA, USA Program in Immunology, Fred Hutchinson Cancer Research Center, Seattle, WA, USA Department of Human Genetics, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA, USA Program in Immunology, Fred Hutchinson Cancer Research Center, Seattle, WA, USA; Department of Medicine, University of Washington, Seattle, WA, USA * Corresponding author; email: ehwarren{at}u.washington.edu. Minor histocompatibility antigens (mHAg) are peptides encoded by polymorphic genes that are presented by MHC molecules and recognized by T cells in recipients of allogeneic hematopoietic cell transplants. Here we report that an alternative transcript of the proliferation associated nuclear element 1 (PANE1) gene encodes a novel HLA-A*0301-restricted mHAg that is selectively expressed in B-lymphoid cells. The antigenic peptide is entirely encoded within a unique exon not present in other PANE1 transcripts. Sequencing of PANE1 alleles in mHAg-positive and mHAg-negative cells demonstrates that differential T cell recognition is due to a single nucleotide polymorphism within the variant exon that replaces an arginine codon with a translation termination codon. The PANE1 transcript that encodes the mHAg is expressed at high levels in resting CD19+ B cells and B-lineage chronic lymphocytic leukemia (B-CLL) cells, and at significantly lower levels in activated B cells. Activation of B-CLL cells through CD40L stimulation decreases expression of the mHAg-encoding PANE1 transcript and reciprocally increases expression of PANE1 transcripts lacking the mHAg-encoding exon. These studies suggest distinct roles for different PANE1 isoforms in resting compared with activated CD19+ cells, and identify PANE1 as a potential therapeutic target in B-CLL. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. N. Stumpf, E. D. van der Meijden, C. A. M. van Bergen, R. Willemze, J. H. F. Falkenburg, and M. Griffioen Identification of 4 new HLA-DR-restricted minor histocompatibility antigens as hematopoietic targets in antitumor immunity Blood, October 22, 2009; 114(17): 3684 - 3692. [Abstract] [Full Text] [PDF] D. S. DeLuca, B. Eiz-Vesper, N. Ladas, B. A.-M. Khattab, and R. 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