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Blood, 1 May 2006, Vol. 107, No. 9, pp. 3772-3778. Prepublished online as a Blood First Edition Paper on January 26, 2006; DOI 10.1182/blood-2005-09-3592. This Article Full Text (PDF) All Versions of this Article: 2005-09-3592v1 107/9/3772    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Larochelle, A. Articles by Hematti, P. Search for Related Content PubMed PubMed Citation Articles by Larochelle, A. Articles by Hematti, P. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted September 6, 2005 Accepted December 19, 2005 AMD3100 mobilizes hematopoietic stem cells with long-term repopulating capacity in non-human primates Andre Larochelle, Allen Krouse, Mark Metzger, Donald Orlic, Robert E Donahue, Simon Fricker, Gary Bridger, Cynthia E Dunbar*, and Peiman Hematti National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA AnorMED Inc., Langley, BC, Canada * Corresponding author; email: dunbarc{at}nhlbi.nih.gov. AMD3100, a bicyclam antagonist of the chemokine receptor CXCR4, has been shown to induce rapid mobilization of CD34+ hematopoietic cells in mice, dogs, and humans, offering an alternative to G-CSF mobilization of peripheral blood hematopoietic stem cells. In this study, AMD3100-mobilized CD34+ cells were phenotypically analyzed, marked with neoR-containing retroviral vectors and subsequently transplanted in myeloablated rhesus macaques. We show engraftment of transduced AMD3100-mobilized CD34+ cells with neoR gene marked myeloid and lymphoid cells up to 32 months after transplantation, demonstrating the ability of AMD3100 to mobilize true long-term repopulating hematopoietic stem cells. More AMD3100-mobilized CD34+ cells are in the G1 phase of the cell cycle and more cells express CXCR4 and VLA-4 compared to G-CSF-mobilized CD34+ cells. In vivo gene marking levels obtained with AMD3100-mobilized CD34+ cells were better than those obtained using CD34+ cells mobilized with G-CSF alone. Overall, these results indicate that AMD3100 mobilizes a population of hematopoietic stem cells with intrinsic characteristics different from those of hematopoietic stem cells mobilized with G-CSF, suggesting fundamental differences in the mechanism of AMD3100-mediated and G-CSF-mediated hematopoietic stem cell mobilization. Thus, AMD3100-mobilized CD34+ cells represent an alternative source of hematopoietic stem cells for clinical stem cell transplantation and genetic manipulation with integrating retroviral vectors. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Mobilization as a preparative regimen for hematopoietic stem cell transplantation Jing Chen, André Larochelle, Simon Fricker, Gary Bridger, Cynthia E. Dunbar, and Janis L. Abkowitz Blood 2006 107: 3764-3771. [Abstract] [Full Text] [PDF] This article has been cited by other articles: R. E. Donahue, P. Jin, A. C. Bonifacino, M. E. Metzger, J. Ren, E. Wang, and D. F. Stroncek Plerixafor (AMD3100) and granulocyte colony-stimulating factor (G-CSF) mobilize different CD34+ cell populations based on global gene and microRNA expression signatures Blood, September 17, 2009; 114(12): 2530 - 2541. [Abstract] [Full Text] [PDF] V. Serikov, C. Hounshell, S. Larkin, W. Green, H. Ikeda, M. C. Walters, and F. A. Kuypers A BRIEF COMMUNICATION: Human Term Placenta as a Source of Hematopoietic Cells Experimental Biology and Medicine, July 1, 2009; 234(7): 813 - 823. [Abstract] [Full Text] [PDF] S. M. Devine, R. Vij, M. Rettig, L. Todt, K. McGlauchlen, N. Fisher, H. Devine, D. C. Link, G. Calandra, G. Bridger, et al. Rapid mobilization of functional donor hematopoietic cells without G-CSF using AMD3100, an antagonist of the CXCR4/SDF-1 interaction Blood, August 15, 2008; 112(4): 990 - 998. [Abstract] [Full Text] [PDF] N. Smart and P. R. Riley The Stem Cell Movement Circ. Res., May 23, 2008; 102(10): 1155 - 1168. [Abstract] [Full Text] [PDF] I. Bruns, U. Steidl, J. C. Fischer, A. Czibere, G. Kobbe, S. Raschke, R. Singh, R. Fenk, M. Rosskopf, S. Pechtel, et al. Pegylated granulocyte colony-stimulating factor mobilizes CD34+ cells with different stem and progenitor subsets and distinct functional properties in comparison with unconjugated granulocyte colony-stimulating factor Haematologica, March 1, 2008; 93(3): 347 - 355. [Abstract] [Full Text] [PDF] A. Zernecke, I. Bot, Y. Djalali-Talab, E. Shagdarsuren, K. Bidzhekov, S. Meiler, R. Krohn, A. Schober, M. Sperandio, O. Soehnlein, et al. Protective Role of CXC Receptor 4/CXC Ligand 12 Unveils the Importance of Neutrophils in Atherosclerosis Circ. Res., February 1, 2008; 102(2): 209 - 217. [Abstract] [Full Text] [PDF] B. E. Shepherd, H.-P. Kiem, P. M. Lansdorp, C. E. Dunbar, G. Aubert, A. LaRochelle, R. Seggewiss, P. Guttorp, and J. L. Abkowitz Hematopoietic stem-cell behavior in nonhuman primates Blood, September 15, 2007; 110(6): 1806 - 1813. [Abstract] [Full Text] [PDF] M. M. Rosenkilde, L.-O. Gerlach, S. Hatse, R. T. Skerlj, D. Schols, G. J. Bridger, and T. W. Schwartz Molecular Mechanism of Action of Monocyclam Versus Bicyclam Non-peptide Antagonists in the CXCR4 Chemokine Receptor J. Biol. Chem., September 14, 2007; 282(37): 27354 - 27365. [Abstract] [Full Text] [PDF] S. Fukuda, H. Bian, A. G. King, and L. M. Pelus The chemokine GRO{beta} mobilizes early hematopoietic stem cells characterized by enhanced homing and engraftment Blood, August 1, 2007; 110(3): 860 - 869. [Abstract] [Full Text] [PDF] S. Forde, B. J. Tye, S. E. Newey, M. Roubelakis, J. Smythe, C. P. McGuckin, R. Pettengell, and S. M. Watt Endolyn (CD164) modulates the CXCL12-mediated migration of umbilical cord blood CD133+ cells Blood, March 1, 2007; 109(5): 1825 - 1833. [Abstract] [Full Text] [PDF] E. E. McCandless, Q. Wang, B. M. Woerner, J. M. Harper, and R. S. Klein CXCL12 Limits Inflammation by Localizing Mononuclear Infiltrates to the Perivascular Space during Experimental Autoimmune Encephalomyelitis J. Immunol., December 1, 2006; 177(11): 8053 - 8064. [Abstract] [Full Text] [PDF] J. Honold, R. Lehmann, C. Heeschen, D. H. Walter, B. Assmus, K.-I. Sasaki, H. Martin, J. Haendeler, A. M. Zeiher, and S. Dimmeler Effects of Granulocyte Colony Stimulating Factor on Functional Activities of Endothelial Progenitor Cells in Patients With Chronic Ischemic Heart Disease Arterioscler Thromb Vasc Biol, October 1, 2006; 26(10): 2238 - 2243. [Abstract] [Full Text] [PDF] J. Chen, A. Larochelle, S. Fricker, G. Bridger, C. E. Dunbar, and J. L. Abkowitz Mobilization as a preparative regimen for hematopoietic stem cell transplantation Blood, May 1, 2006; 107(9): 3764 - 3771. [Abstract] [Full Text] [PDF]

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