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Blood, 1 May 2006, Vol. 107, No. 9, pp. 3764-3771. Prepublished online as a Blood First Edition Paper on January 26, 2006; DOI 10.1182/blood-2005-09-3593. This Article Full Text (PDF) All Versions of this Article: 2005-09-3593v1 107/9/3764    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Chen, J. Articles by Abkowitz, J. L Search for Related Content PubMed PubMed Citation Articles by Chen, J. Articles by Abkowitz, J. L Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted September 6, 2005 Accepted December 8, 2005 Mobilization as a preparative regimen for hematopoietic stem cell transplantation Jing Chen, Andre Larochelle, Simon Fricker, Gary Bridger, Cynthia E Dunbar, and Janis L Abkowitz* Medicine/Hematology, University of Washington, Seattle, WA, USA Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA AnorMED Inc., Langley, BC, Canada * Corresponding author; email: janabk{at}u.washington.edu. Current myeloablative conditioning regimens for hematopoietic stem cell (HSC) transplantation are associated with significant morbidity and mortality. Thus, alternative strategies to promote engraftment of infused HSCs with increased safety warrant investigation. Using parabiotic mice, we determined that, after mobilization with AMD3100 (a CXCR4 antagonist), HSCs exited from marrow, transited blood, and engrafted in open niches in partner marrow. We then hypothesized that mobilization before transplantation might vacate niches and improve HSC engraftment. When PeP3b mice were treated with AMD3100 2h before the transplantation of 4 X 107 marrow cells, donor cell engraftment was higher (4.6% ± 1.1%) than in control animals (no AMD3100) (1.0% ± 0.24%, p=0.0004). When mice received weekly injections of AMD3100 on three consecutive weeks and marrow cells were transplanted 2 hours after each mobilization, donor cell engraftment further increased (9.1% ± 1.7%, p=0.001). In contrast, in similar experiments with Balb/cByJ mice that mobilize poorly, there was no difference between the donor cell engraftment of AMD3100-treated and control recipients. These results indicate that the number of available niches regulates the number of HSCs. In addition, mobilization with AMD3100 may provide a safer preparative approach for HSC transplantation in genetic and other nonmalignant disorders. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: AMD3100 mobilizes hematopoietic stem cells with long-term repopulating capacity in nonhuman primates André Larochelle, Allen Krouse, Mark Metzger, Donald Orlic, Robert E. Donahue, Simon Fricker, Gary Bridger, Cynthia E. Dunbar, and Peiman Hematti Blood 2006 107: 3772-3778. [Abstract] [Full Text] [PDF] This article has been cited by other articles: J. L. Abkowitz and J. Chen Studies of c-Mpl function distinguish the replication of hematopoietic stem cells from the expansion of differentiating clones Blood, June 15, 2007; 109(12): 5186 - 5190. [Abstract] [Full Text] [PDF] C. Barese, N. Pech, S. Dirscherl, J. L. Meyers, A. L. Sinn, M. C. Yoder, W. S. Goebel, and M. C. Dinauer Granulocyte Colony-Stimulating Factor Prior to Nonmyeloablative Irradiation Decreases Murine Host Hematopoietic Stem Cell Function and Increases Engraftment of Donor Marrow Cells Stem Cells, June 1, 2007; 25(6): 1578 - 1585. [Abstract] [Full Text] [PDF]

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