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Blood, 1 June 2006, Vol. 107, No. 11, pp. 4317-4325. Prepublished online as a Blood First Edition Paper on February 2, 2006; DOI 10.1182/blood-2005-09-3747. This Article Full Text (PDF) Supplemental Figures All Versions of this Article: 2005-09-3747v1 107/11/4317    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Takayanagi, S.-i. Articles by Nakauchi, H. Search for Related Content PubMed PubMed Citation Articles by Takayanagi, S.-i. Articles by Nakauchi, H. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted September 21, 2005 Accepted January 22, 2006 Genetic marking of hematopoietic stem and endothelial cells: identification of the Tmtsp gene encoding a novel cell-surface protein with the thrombospondin-1 domain Shin-ichiro Takayanagi, Takashi Hiroyama, Satoshi Yamazaki, Tomoko Nakajima, Yohei Morita, Joichi Usui, Koji Eto, Tsutomu Motohashi, Kensuke Shiomi, Kazuko Keino-Masu, Masayuki Masu, Yuichi Oike, Shigeo Mori, Nobuaki Yoshida, Atsushi Iwama*, and Hiromitsu Nakauchi Laboratory of Stem Cell Therapy, Center for Experimental Medicine, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo, Japan Cell Engineering Division, RIKEN BioResource Center, Ibaraki, Japan Department of Cancer Research Division of Pathology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo, Japan Department of Tissue and Organ Development, Regeneration and Advanced Medical Science, Gifu University Graduate School of Medicine, Gifu, Japan Department of Molecular Neurobiology, Institute of Basic Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan Department of Cell Differentiation, The Sakaguchi Laboratory, School of Medicine, Keio University, Shinjuku-ku, Tokyo, Japan Division of Gene Expression and Regulation, Center for Experimental Medicine, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo, Japan Laboratory of Stem Cell Therapy, Center for Experimental Medicine, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo, Japan; Department of Cellular and Molecular Medicine, Graduate School of Medicine, Chiba University, Chiba, Japan * Corresponding author; email: aiwama{at}faculty.chiba-u.jp. Using an in silico database search, we identified a novel gene encoding a cell-surface molecule with a thrombospondin domain, and designated the gene as Transmembrane Molecule with Thrombospondin Module (Tmtsp). Expression profiling of Tmtsp using specific monoclonal antibodies and Venus, a variant of yellow fluorescent protein "knock-in" mice in the Tmtsp locus demonstrated its specific expression in hematopoietic and endothelial cells. In lymphohematopoietic cells, Tmtsp was predominantly expressed in hematopoietic stem and progenitor cells and the level of expression gradually declined as the cells differentiated. Venus expression faithfully traced the expression of Tmtsp and the level of Venus expression correlated well to the in vitro hematopoietic activity as well as the in vivo bone marrow repopulating capacity. Notably, Venus expression marked the development of definitive hematopoiesis in both the extraembryonic yolk sac and the intraembryonic aorta-gonad-mesonephros (AGM) region, and in combination with CD41, strikingly promoted the enrichment of developing progenitors in the CD41+Venushigh fraction at embryonic day 10.5 (E10.5). In this context, Tmtsp is a novel marker gene for primitive hematopoietic cells and endothelial cells, and TmtspVenus/+ mice would serve as a valuable mouse model for the analysis of both embryonic and adult hematopoiesis, as well as for vascular biology. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

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