SEARCH:   Advanced

Blood, 1 August 2006, Vol. 108, No. 3, pp. 896-903. Prepublished online as a Blood First Edition Paper on April 4, 2006; DOI 10.1182/blood-2005-09-3846. This Article Full Text (PDF) Supplemental Table and Figure All Versions of this Article: 2005-09-3846v1 108/3/896    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sakamoto, H. Articles by Ogawa, M. Search for Related Content PubMed PubMed Citation Articles by Sakamoto, H. Articles by Ogawa, M. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted September 26, 2005 Accepted March 23, 2006 Proper levels of c-Myb are discretely defined at distinct steps of hematopoietic cell development Hiroshi Sakamoto, Guoyou Dai, Kaori Tsujino, Kazuaki Hashimoto, Xin Huang, Tetsuhiro Fujimoto, Michael Mucenski, Jon Frampton, and Minetaro Ogawa* Department of Cell Differentiation, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan Department of Cell Differentiation, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan; The 21st Century COE "Cell Fate Regulation Research and Education Unit", Kumamoto University, Kumamoto, Japan Division of Pulmonary Biology, Children's Hospital Medical Center, Cincinnati, OH, USA Institute for Biomedical Research, The Medical School, Birmingham University, Birmingham, United Kingdom * Corresponding author; email: minetaro{at}kaiju.medic.kumamoto-u.ac.jp. The definitive hematopoietic cell lineages have been proposed to originate from hemogenic endothelial cells during mouse embryogenesis. c-Myb is a transcription factor that is essential for the development of definitive hematopoiesis. To investigate the functional role of c-Myb in hematopoietic cell development from endothelial cells, we introduced a c-myb transgene expressed under the control of a tetracycline-regulated promoter into the c-myb-/- ES cell line, with the aim of inducing c-Myb expression at any stage and at any level. Induction of c-Myb expression after replating c-myb-/- endothelial cells rescued the generation and proliferation of definitive hematopoietic progenitor cells, suggesting that c-Myb expression in developing endothelial cells is not a prerequisite for their hematogenic potential. Over expression of c-Myb, however, prevented the terminal differentiation of erythrocytes and megakaryocytes, and completely abolished B lymphocyte development. Our results indicate that c-Myb is a major factor that controls differentiation as well as proliferation of hematopoietic progenitor cells derived from hemogenic endothelial cells, and that appropriate levels of c-Myb protein are strictly defined at distinct differentiation steps of each hematopoietic cell lineage. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. P. Fahl, R. B. Crittenden, D. Allman, and T. P. Bender c-Myb Is Required for Pro-B Cell Differentiation J. Immunol., November 1, 2009; 183(9): 5582 - 5592. [Abstract] [Full Text] [PDF] M. Matsukawa, H. Sakamoto, M. Kawasuji, T. Furuyama, and M. Ogawa Different roles of Foxo1 and Foxo3 in the control of endothelial cell morphology Genes Cells, October 1, 2009; 14(10): 1167 - 1181. [Abstract] [Full Text] [PDF] C-C So, Y-Q Song, S T Tsang, L-F Tang, A Y Chan, E S Ma, and L-C Chan The HBS1L-MYB intergenic region on chromosome 6q23 is a quantitative trait locus controlling fetal haemoglobin level in carriers of {beta}-thalassaemia J. Med. Genet., November 1, 2008; 45(11): 745 - 751. [Abstract] [Full Text] [PDF] K. M. Kolodziejska, M.H. Noyan-Ashraf, A. Nagy, A. Bacon, J. Frampton, H.-B. Xin, M. I. Kotlikoff, and M. Husain c-Myb-Dependent Smooth Muscle Cell Differentiation Circ. Res., March 14, 2008; 102(5): 554 - 561. [Abstract] [Full Text] [PDF] J. O'Neil, J. Tchinda, A. Gutierrez, L. Moreau, R. S. Maser, K.-K. Wong, W. Li, K. McKenna, X. S. Liu, B. Feng, et al. Alu elements mediate MYB gene tandem duplication in human T-ALL J. Exp. Med., December 24, 2007; 204(13): 3059 - 3066. [Abstract] [Full Text] [PDF] E. Clappier, W. Cuccuini, A. Kalota, A. Crinquette, J.-M. Cayuela, W. A. Dik, A. W. Langerak, B. Montpellier, B. Nadel, P. Walrafen, et al. The C-MYB locus is involved in chromosomal translocation and genomic duplications in human T-cell acute leukemia (T-ALL), the translocation defining a new T-ALL subtype in very young children Blood, August 15, 2007; 110(4): 1251 - 1261. [Abstract] [Full Text] [PDF] M. Sramko, J. Markus, J. Kabat, L. Wolff, and J. Bies Stress-induced Inactivation of the c-Myb Transcription Factor through Conjugation of SUMO-2/3 Proteins J. Biol. Chem., December 29, 2006; 281(52): 40065 - 40075. [Abstract] [Full Text] [PDF]

	Copyright © 2006 by American Society of Hematology         Online ISSN: 1528-0020