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Blood, 1 May 2006, Vol. 107, No. 9, pp. 3745-3752. Prepublished online as a Blood First Edition Paper on December 29, 2005; DOI 10.1182/blood-2005-10-4094. This Article Full Text (PDF) All Versions of this Article: 2005-10-4094v1 107/9/3745    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Buffet, P. A Articles by David, P. H Search for Related Content PubMed PubMed Citation Articles by Buffet, P. A Articles by David, P. H Social Bookmarking                   What's this?  Previous Article Submitted October 13, 2005 Accepted December 20, 2005 Ex-vivo perfusion of human spleens maintains clearing and processing functions Pierre A Buffet*, Genevieve Milon, Valentine Brousse, Jean-Michel Correas, Bertrand Dousset, Anne Couvelard, Reza Kianmanesh, Olivier Farges, Alain Sauvanet, Francois Paye, Marie-Noelle Ungeheuer, Catherine Ottone, Huot Khun, Laurence Fiette, Ghislaine Guigon, Michel Huerre, Odile Mercereau-Puijalon, and Peter H David Biomedical Research Team, Medical Center, Institut Pasteur, Paris, France; Molecular Immunology of Parasites Unit, CNRS URA 2581, Parasitology Department, Institut Pasteur, Paris, France Immunophysiology and Intracellular Parasitism Unit, Parasitology Department, Institut Pasteur, Paris, France Assistance Publique - Hopitaux de Paris, Paris, France Biomedical Research Team, Medical Center, Institut Pasteur, Paris, France URE Histotechnology and Pathology, Institut Pasteur, Paris, France Public Health Platform, Institut Pasteur, Paris, France Molecular Immunology of Parasites Unit, CNRS URA 2581, Parasitology Department, Institut Pasteur, Paris, France * Corresponding author; email: pabuffet{at}pasteur.fr. The spleen plays a central role in the pathophysiology of several potentially severe diseases such as inherited red cell membrane disorders, hemolytic anemias and malaria. Research on these diseases is hampered by ethical constraints that limit human spleen tissue explorations. We identified a surgical situation - left spleno-pancreatectomy for benign pancreas tumours - allowing spleen retrieval at no risk for patients. Ex-vivo perfusion of retrieved intact spleens during 4-6 hours preserved parenchymal structure, vascular flow and metabolic activity. Function preservation was assessed by testing the ability of isolated-perfused spleens to retain Plasmodium falciparum - infected erythrocytes pre-exposed to the antimalarial drug artesunate (Art-iRBCs). More than 95% of Art-iRBCs were cleared from the perfusate in 2 hours. At each transit through isolated-perfused spleens, parasite remnants were removed from 0.2% - 0.23% of Art-iRBCs, a proportion consistent with the 0.02% - 1% pitting rate previously established in artesunate-treated patients. Histological analysis showed that > 90% of Art-iRBCs were retained and processed in the red pulp, providing the first direct evidence of a zone-dependent parasite clearance by the human spleen. Human-specific physiological or pathophysiological mechanisms involving clearing or processing functions of the spleen can now be experimentally explored in a human tissue context. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: I. Safeukui, J.-M. Correas, V. Brousse, D. Hirt, G. Deplaine, S. Mule, M. Lesurtel, N. Goasguen, A. Sauvanet, A. Couvelard, et al. Retention of Plasmodium falciparum ring-infected erythrocytes in the slow, open microcirculation of the human spleen Blood, September 15, 2008; 112(6): 2520 - 2528. [Abstract] [Full Text] [PDF] M. Mamani-Matsuda, A. Cosma, S. Weller, A. Faili, C. Staib, L. Garcon, O. Hermine, O. Beyne-Rauzy, C. Fieschi, J.-O. Pers, et al. The human spleen is a major reservoir for long-lived vaccinia virus-specific memory B cells Blood, May 1, 2008; 111(9): 4653 - 4659. [Abstract] [Full Text] [PDF]

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