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Blood, 15 May 2006, Vol. 107, No. 10, pp. 3865-3867. Prepublished online as a Blood First Edition Paper on January 26, 2006; DOI 10.1182/blood-2005-10-4108. This Article Full Text (PDF) Supplemental Figures All Versions of this Article: 2005-10-4108v1 107/10/3865    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Seggewiss, R. Articles by Dunbar, C. E Search for Related Content PubMed PubMed Citation Articles by Seggewiss, R. Articles by Dunbar, C. E Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted October 14, 2005 Accepted January 13, 2006 Acute myeloid leukemia associated with retroviral gene transfer to hematopoietic progenitor cells of a rhesus macaque Ruth Seggewiss, Stefania Pittaluga, Rima L Adler, F J Guenaga, Cole Ferguson, Ingo Pilz, Byoung Ryu, Brian P Sorrentino, W S Young, Robert E Donahue, Christof von Kalle, Arthur W Nienhuis, and Cynthia E Dunbar* Hematology Branch, National Heart, Lung, and Blood Institute, NIH, Bethesda, MD, USA Hematopathology Section, National Cancer Institute, NIH, Bethesda, MD, USA Department of Medicine I, University of Freiburg, Freiburg, Germany Division of Experimental Hematology, St. Jude Children's Research Hospital, Division of Experimental Hematology, Memphis, TN, USA Section on Neural Gene Expression, National Institute of Mental Health, NIH/DHHS, Bethesda, MD, USA Department of Medicine I, University of Freiburg, Freiburg, Germany; Molecular and Gene Therapy Program, Children's Hospital Research Foundation, Cincinnati, OH, USA * Corresponding author; email: dunbarc{at}nhlbi.nih.gov. We report for the first time a replication-defective retroviral vector-associated neoplasia in a non-human primate. Five years after transplantation with CD34+ cells transduced with a retroviral vector expressing eGFP and a drug-resistant variant of the dihydrofolate reductase gene, a rhesus macaque developed a fatal myeloid sarcoma, a type of acute myeloid leukemia. Tumor cells contained two clonal vector insertions. One insertion was found in BCL-2A1, an anti-apoptotic gene. This event suggests that currently available retroviral vectors may have long-term side effects particularly in hematopoietic stem and progenitor cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: K. J. POSEKANY, J. E. WILEY, and G. A. GAGNON A Novel Method to Display [gal {alpha}1, 3 gal] Antigens on Human Leukemic Cells for Preparation of Anti-leukemia Vaccines Anticancer Res, June 1, 2009; 29(6): 2387 - 2392. 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