SEARCH:   Advanced

Blood, 15 April 2006, Vol. 107, No. 8, pp. 3122-3130. Prepublished online as a Blood First Edition Paper on January 10, 2006; DOI 10.1182/blood-2005-10-4120. This Article Full Text (PDF) Supplemental Table All Versions of this Article: 2005-10-4120v1 107/8/3122    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Willey, S. Articles by Baron, M. H Search for Related Content PubMed PubMed Citation Articles by Willey, S. Articles by Baron, M. H Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted October 17, 2005 Accepted December 8, 2005 Acceleration of mesoderm development and expansion of hematopoietic progenitors in differentiating ES cells by the mouse mix-like homeodomain transcription factor Stephen Willey, Angel Ayuso-Sacido, Hailan Zhang, Stuart T Fraser, Kenneth E Sahr, Matthew J Adlam, Michael Kyba, George Q Daley, Gordon Keller, and Margaret H Baron* Department of Medicine, Mount Sinai School of Medicine, New York, NY, USA Center for Developmental Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA Division of Hematology/Oncology, Children's Hospital, Boston, MA, USA Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY, USA Department of Medicine, Mount Sinai School of Medicine, New York, NY, USA; Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY, USA; Department of Oncological Sciences and Molecular, Cellular and Developmental Biology, Mount Sinai School of Medicine, New York, NY, USA * Corresponding author; email: margaret.baron{at}mssm.edu. The cellular and molecular events underlying the formation and differentiation of mesoderm to derivatives such as blood are critical to our understanding of the development and function of many tissues and organ systems. How different mesodermal populations are set aside to form specific lineages is not well understood. Although previous genetic studies in the mouse embryo have pointed to a critical role for the homeobox gene Mix-like (mMix) in gastrulation, its function in mesoderm development remains unclear. Hematopoietic defects have been identified in differentiating embryonic stem cells in which mMix was genetically inactivated. Here we show that conditional induction of mMix in embryonic stem cell-derived embryoid bodies results in the early activation of mesodermal markers prior to expression of Brachury/T and acceleration of the mesodermal developmental program. Strikingly, increased numbers of mesodermal, hemangioblastic, and hematopoietic progenitors form in response to premature activation of mMix. Differentiation to primitive (embryonic) and definitive (adult type) blood cells proceeds normally and without an apparent bias in the representation of different hematopoietic cell fates. Therefore, the mouse Mix gene functions early in the recruitment and/or expansion of mesodermal progenitors to the hemangioblastic and hematopoietic lineages. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: R. N. Re and J. L. Cook The physiological basis of intracrine stem cell regulation Am J Physiol Heart Circ Physiol, August 1, 2008; 295(2): H447 - H453. [Abstract] [Full Text] [PDF] F. Lohmann and J. J. Bieker Activation of Eklf expression during hematopoiesis by Gata2 and Smad5 prior to erythroid commitment Development, June 15, 2008; 135(12): 2071 - 2082. [Abstract] [Full Text] [PDF] C. Wang, P. W. Faloon, Z. Tan, Y. Lv, P. Zhang, Y. Ge, H. Deng, and J.-W. Xiong Mouse lysocardiolipin acyltransferase controls the development of hematopoietic and endothelial lineages during in vitro embryonic stem-cell differentiation Blood, November 15, 2007; 110(10): 3601 - 3609. [Abstract] [Full Text] [PDF] R. C. R. Perlingeiro Endoglin is required for hemangioblast and early hematopoietic development Development, August 15, 2007; 134(16): 3041 - 3048. [Abstract] [Full Text] [PDF] H. K. Bone and M. J. Welham Phosphoinositide 3-kinase signalling regulates early development and developmental haemopoiesis J. Cell Sci., May 15, 2007; 120(10): 1752 - 1762. [Abstract] [Full Text] [PDF] H. Zhang and M. H. Baron The Hematopoietic Regulator Mouse Mix Functions as a Transcriptional Activator in Embryonic Stem Cells. Blood (ASH Annual Meeting Abstracts), November 16, 2006; 108(11): 1189 - 1189. [Abstract] [PDF] S. Glaser, D. Metcalf, L. Wu, A. H. Hart, L. DiRago, S. Mifsud, A. D'Amico, S. Dagger, C. Campo, A. C. Chan, et al. Enforced expression of the homeobox gene Mixl1 impairs hematopoietic differentiation and results in acute myeloid leukemia PNAS, October 31, 2006; 103(44): 16460 - 16465. [Abstract] [Full Text] [PDF] X. Li, J.-W. Xiong, C. S. Shelley, H. Park, and M. A. Arnaout The transcription factor ZBP-89 controls generation of the hematopoietic lineage in zebrafish and mouse embryonic stem cells Development, September 15, 2006; 133(18): 3641 - 3650. [Abstract] [Full Text] [PDF] L.-C. Wu, C.-W. Sun, T. M. Ryan, K. M. Pawlik, J. Ren, and T. M. Townes Correction of sickle cell disease by homologous recombination in embryonic stem cells Blood, August 15, 2006; 108(4): 1183 - 1188. [Abstract] [Full Text] [PDF]

	Copyright © 2006 by American Society of Hematology         Online ISSN: 1528-0020