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Blood, 15 July 2006, Vol. 108, No. 2, pp. 737-744. Prepublished online as a Blood First Edition Paper on March 28, 2006; DOI 10.1182/blood-2005-10-4135. This Article Full Text (PDF) Supplemental Figures All Versions of this Article: 2005-10-4135v1 108/2/737    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kim, I. Articles by Morrison, S. J Search for Related Content PubMed PubMed Citation Articles by Kim, I. Articles by Morrison, S. J Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted October 18, 2005 Accepted March 11, 2006 Enhanced purification of fetal liver hematopoietic stem cells using SLAM family receptors Injune Kim, Shenghui He, Omer H Yilmaz, Mark J Kiel, and Sean J Morrison* Howard Hughes Medical Institute, Life Sciences Institute, Department of Internal Medicine, Center for Stem Cell Biology, University of Michigan, Ann Arbor, MI, USA * Corresponding author; email: seanjm{at}umich.edu. Although adult mouse hematopoietic stem cells (HSCs) have been purified to near homogeneity it remains impossible to achieve this with fetal HSCs. Adult HSC purity has recently been enhanced using the SLAM family receptors CD150, CD244, and CD48. These markers are expressed at different stages of the hematopoiesis hierarchy, making it possible to highly purify adult HSCs as CD150+CD48-CD244- cells. We found that SLAM family receptors exhibited a similar expression pattern in fetal liver. Fetal liver HSCs were CD150+CD48-CD244- and the vast majority of colony-forming progenitors were CD48+CD244-CD150- or CD48+CD244+CD150-, just as in adult bone marrow. SLAM family markers enhanced the purification of fetal liver HSCs. Whereas 1 out of every 8.9 (11%) ThylowSca-1+lineage-Mac-1+ fetal liver cells gave long-term multilineage reconstitution in irradiated mice, 1 out of every 5.7 (18%) CD150+CD48-CD41- cells and 1 out of every 2.7 (37%) CD150+CD48-Sca-1+lineage-Mac-1+ fetal liver cells gave long-term multilineage reconstitution. These data emphasize the robustness with which SLAM family markers distinguish progenitors at different stages of the hematopoiesis hierarchy and enhance the purification of definitive HSCs from diverse contexts. Nonetheless, CD150, CD244, and CD48 are not pan-stem cell markers as they were not detectably expressed by stem cells in the fetal or adult nervous system. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: R. XuFeng, M. J. Boyer, H. Shen, Y. Li, H. Yu, Y. Gao, Q. Yang, Q. Wang, and T. Cheng ADAR1 is required for hematopoietic progenitor cell survival via RNA editing PNAS, October 20, 2009; 106(42): 17763 - 17768. [Abstract] [Full Text] [PDF] S. L. McKinney-Freeman, O. Naveiras, F. Yates, S. Loewer, M. Philitas, M. Curran, P. J. Park, and G. Q. Daley Surface antigen phenotypes of hematopoietic stem cells from embryos and murine embryonic stem cells Blood, July 9, 2009; 114(2): 268 - 278. [Abstract] [Full Text] [PDF] N. Buza-Vidas, M. Cheng, S. Duarte, H. Nozad Charoudeh, S. E. W. Jacobsen, and E. 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