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Blood, 1 May 2006, Vol. 107, No. 9, pp. 3527-3530. Prepublished online as a Blood First Edition Paper on January 3, 2006; DOI 10.1182/blood-2005-10-4309. This Article Full Text (PDF) Supplemental Figure All Versions of this Article: 2005-10-4309v1 107/9/3527    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Rooke, H. M Articles by Orkin, S. H Search for Related Content PubMed PubMed Citation Articles by Rooke, H. M Articles by Orkin, S. H Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted November 3, 2005 Accepted December 16, 2005 Phosphorylation of Gata1 at serine residues 72, 142 and 310 is not essential for hematopoiesis in vivo Heather M Rooke and Stuart H Orkin* Division of Hematology/Oncology, Children's Hospital Boston and the Dana Farber Cancer Institute, Boston, MA, USA; Harvard Medical School, Boston, MA, USA; Howard Hughes Medical Institute, Boston, MA, USA Division of Hematology/Oncology, Children's Hospital Boston and the Dana Farber Cancer Institute, Boston, MA, USA; Howard Hughes Medical Institute, Boston, MA, USA * Corresponding author; email: orkin{at}bloodgroup.tch.harvard.edu. Phosphorylation of transcription factors is important in post-translational control of protein function. The indispensable zinc-finger transcription factor, Gata1, is phosphorylated constitutively at six serine residues (26, 49, 72, 142, 178, 187) and at a seventh (310) following induction of erythroid differentiation. However, the biological consequences of phosphorylation with respect to function are unclear. To address this issue, we generated mice with serine to alanine mutations at the inducibly-phosphorylated serine 310 alone or at conserved serine residues 72, 142 and 310 together. The peripheral blood parameters of the mice were normal, as was their response to acute erythropoietic stress. Analysis of hematopoietic progenitor populations during ontogeny and into adulthood showed a moderate decrease in BFU-E and CFU-E numbers only in the adult bone marrow of the triple mutant. Yet, later stage erythropoiesis was not perturbed. This suggests that any molecular consequences associated with loss of phosphorylation at residues 72, 142 and 310 can be compensated for in the in vivo environment. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. Elorza, B. Hyde, H. K. Mikkola, S. Collins, and O. S. Shirihai UCP2 Modulates Cell Proliferation through the MAPK/ERK Pathway during Erythropoiesis and Has No Effect on Heme Biosynthesis J. Biol. Chem., November 7, 2008; 283(45): 30461 - 30470. [Abstract] [Full Text] [PDF]

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