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Blood, 1 July 2006, Vol. 108, No. 1, pp. 203-208. Prepublished online as a Blood First Edition Paper on February 14, 2006; DOI 10.1182/blood-2005-11-4330. This Article Full Text (PDF) All Versions of this Article: 2005-11-4330v1 108/1/203    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Abbasian, J. Articles by Rondelli, D. Search for Related Content PubMed PubMed Citation Articles by Abbasian, J. Articles by Rondelli, D. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted November 2, 2005 Accepted February 6, 2006 Allogeneic t cells induce rapid cd34+ cell differentiation into cd11c+cd86+ cells with direct and indirect antigen-presenting function Javaneh Abbasian, Dolores Mahmud, Nadim Mahmud, Sandeep Chunduri, Hiroto Araki, Pavan Reddy, Ronald Hoffman, Mario Arpinati, James L Ferrara, and Damiano Rondelli* Section of Hematology/Oncology, University of Illinois at Chicago, Chicago, Illinois, USA Departments of Pediatrics and Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan, USA Institute of Hematology and Medical Oncology Seragnoli, University of Bologna, Bologna, Italy * Corresponding author; email: drond{at}uic.edu. Dendritic cells (DC) derive from CD34+ cells or monocytes and stimulate alloimmune responses in transplantation. We hypothesized that the interaction between CD34+ cells and allogeneic T-cells would influence the function of hematopoietic stem cells (HSC). Cord blood (CB) CD34+ cells proliferated briskly in response to allogeneic, but not autologous, T-cells when mixed with irradiated T-cells for 6 days in-vitro. This proliferation was significantly inhibited by an anti- HLA-class II monoclonal antibody (mAb), by an anti-TNFmAb, or by CTLA4-Ig. Allogeneic T-cells induced the differentiation of CD34+ progenitors into cells with the morphology of dendritic monocytic precursors and characterized by the expression of HLA-DR, CD86, CD40, CD14, CD11c, due to an endogenous release of TNF. Co-transplantation of CD34+ cells with allogeneic T-cells into NOD/SCID mice resulted in a greater engraftment of myeloid CD1c+ dendritic cells, compared to co-transplantation with autologous T-cells. In-vitro, CD34+ cells-derived antigen-presenting cells (APCs) were functionally capable of both direct and indirect presentation of alloantigens. Based on these findings, we hypothesize that in HSC transplantation the initial crosstalk between allogeneic T-cells and CD34+ cells may result in the increased generation of APCs that can present host alloantigens, and possibly contribute to the development of graft-vesus-host disease. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

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