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Blood, 15 July 2006, Vol. 108, No. 2, pp. 487-492. Prepublished online as a Blood First Edition Paper on January 12, 2006; DOI 10.1182/blood-2005-11-4388. This Article Full Text (PDF) All Versions of this Article: 2005-11-4388v1 108/2/487    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lan, P. Articles by Yang, Y.-G. Search for Related Content PubMed PubMed Citation Articles by Lan, P. Articles by Yang, Y.-G. Related Collections Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted November 7, 2005 Accepted December 25, 2005 Reconstitution of a functional human immune system in immunodeficient mice through combined human fetal thymus/liver and CD34+ cell transplantation Ping Lan, Noriko Tonomura, Akira Shimizu, Shumei Wang, and Yong-Guang Yang* Transplantation Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA * Corresponding author; email: yongguang.yang{at}tbrc.mgh.harvard.edu. Studies of the human immune system have been limited by the lack of an appropriate in vivo model. For this reason, efforts have been made to develop murine models with a functional human immune system. We report here that co-transplantation of human fetal thymus/liver tissues and CD34+ hematopoietic stem/progenitor cells lead to the development of sustained human hematopoiesis and a functional human immune system in immunodeficient NOD/SCID mice. The humanized mice showed systemic repopulation with a comprehensive array of human lymphohematopoietic cells, including T cells, B cells, and dendritic cells, and the formation of secondary lymphoid organs. Furthermore, these mice produce high levels of human IgM and IgG antibodies and mediate strong immune responses in vivo as demonstrated by skin xenograft rejection. Thus, the humanized NOD/SCID mice described in this paper provide a powerful model system to study human immune function. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Boosting functional hematolymphopoiesis in humanized mice Tao Cheng Blood 2006 108: 411-412. [Full Text] [PDF] This article has been cited by other articles: D. M. Brainard, E. Seung, N. Frahm, A. Cariappa, C. C. Bailey, W. K. Hart, H.-S. Shin, S. F. Brooks, H. L. Knight, Q. Eichbaum, et al. Induction of Robust Cellular and Humoral Virus-Specific Adaptive Immune Responses in Human Immunodeficiency Virus-Infected Humanized BLT Mice J. Virol., July 15, 2009; 83(14): 7305 - 7321. [Abstract] [Full Text] [PDF] N. Tonomura, K. Habiro, A. Shimizu, M. Sykes, and Y.-G. Yang Antigen-specific human T-cell responses and T cell-dependent production of human antibodies in a humanized mouse model Blood, April 15, 2008; 111(8): 4293 - 4296. [Abstract] [Full Text] [PDF]

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