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Blood, 1 September 2006, Vol. 108, No. 5, pp. 1555-1561. Prepublished online as a Blood First Edition Paper on March 23, 2006; DOI 10.1182/blood-2005-11-4599. This Article Full Text (PDF) All Versions of this Article: 2005-11-4599v1 108/5/1555    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Salmi, M. Articles by Jalkanen, S. Search for Related Content PubMed PubMed Citation Articles by Salmi, M. Articles by Jalkanen, S. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted November 22, 2005 Accepted March 12, 2006 Developmental regulation of the adhesive and enzymatic activity of vascular adhesion protein-1 (VAP-1) in humans Marko Salmi* and Sirpa Jalkanen National Public Health Institute, University of Turku, Turku, Finland; Turku and MediCity Research Laboratory, University of Turku, Turku, Finland * Corresponding author; email: marko.salmi{at}utu.fi. Vascular adhesion protein-1 (VAP-1) is a homodimeric glycoprotein that belongs to a unique subgroup of cell-surface expressed oxidases. In adults endothelial VAP-1 supports leukocyte rolling, firm adhesion and transmigration in both enzyme activity dependent and enzyme activity independent manner. Here we studied the induction and function of VAP-1 during human ontogeny. We show that VAP-1 is already found in the smooth muscle at embryonic week 7. There are marked time-dependent switches in VAP-1 expression in sinusoids of liver, in peritubular capillaries of kidney, in capillaries of heart and in venules in lamina propria of the gut. Fetal VAP-1 is dimerized, and it is enzymatically active. VAP-1 in fetal-type venules is able to bind cord blood lymphocytes. Also, adenovirally transfected VAP-1 on human umbilical vein endothelial cells is involved in rolling and firm adhesion of cord blood lymphocytes under conditions of physiological shear stress. We conclude that VAP-1 is synthesized from early on in human vessels and it is functionally intact already before birth. Thus, VAP-1 may contribute critically to the oxidase activities in utero, and prove important for lymphocyte trafficking during human ontogeny. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

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