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Blood, 1 August 2006, Vol. 108, No. 3, pp. 1037-1044. Prepublished online as a Blood First Edition Paper on March 30, 2006; DOI 10.1182/blood-2005-12-4916. This Article Full Text (PDF) Supplemental Figure and Table All Versions of this Article: 2005-12-4916v1 108/3/1037    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ogata, K. Articles by Hayashi, A. Search for Related Content PubMed PubMed Citation Articles by Ogata, K. Articles by Hayashi, A. Social Bookmarking                   What's this?  Previous Article  |  Next Article  Submitted December 13, 2005 Accepted March 16, 2006 Diagnostic application of flow cytometric characteristics of CD34+ cells in low-grade myelodysplastic syndromes Kiyoyuki Ogata*, Yoshifumi Kishikawa, Chikako Satoh, Hideto Tamura, Kazuo Dan, and Akio Hayashi Division of Hematology, Department of Medicine, Nippon Medical School, Tokyo, Japan Department of Research and Development, Mitsubishi Kagaku Bio-Clinical Laboratories, Tokyo, Japan Division of Hematology, Department of Medicine, Nippon Medical School, Tokyo, Japan; Department of Bioregulation, Institute of Gerontology, Department of Medicine, Nippon Medical School, Kanagawa, Japan * Corresponding author; email: ogata{at}nms.ac.jp. The diagnosis of myelodysplastic syndromes (MDS) without an increase in blasts and ringed sideroblasts (low-grade MDS without ringed sideroblasts [LGw/oRS]) may be problematic because dysplastic features are not specific to MDS and about 50% of LGw/oRS patients lack chromosomal aberrations. Here, we report the utility of flow cytometric characteristics of CD34+ cells for LGw/oRS diagnosis. Bone marrow cells from LGw/oRS patients and controls (e.g., cytopenic individuals without MDS) were analyzed using four-color flow cytometry (FCM). We objectively determined reference ranges of 13 parameters relating to CD34+ cells using data from controls. In LGw/oRS patients, various abnormalities of CD34+ cells were observed, e.g., a decrease in CD34+ B-cell precursors and aberrant or overexpression of various antigens on CD34+ myeloblasts. Then, we constructed a reproducible, flow cytometric scoring system for LGw/oRS diagnosis. High scores were observed in 16 of 27 LGw/oRS patients, regardless of the presence or absence of chromosomal aberrations, but not in any of 90 controls. Among LGw/oRS patients with chromosomal aberrations, patients with trisomy 8 or del20(q) were notably associated with a low FCM score (P = .0019). As a result, most LGw/oRS patients were identified based on a high FCM score and/or chromosomal aberrations. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. A. van de Loosdrecht, T. M. Westers, A. H. Westra, A. M. Drager, V. H. J. van der Velden, and G. J. Ossenkoppele Identification of distinct prognostic subgroups in low- and intermediate-1-risk myelodysplastic syndromes by flow cytometry Blood, February 1, 2008; 111(3): 1067 - 1077. [Abstract] [Full Text] [PDF]

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